Recombinant Porcine IL-12 Protein

Catalog #: 912-PL
7 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
912-PL-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
912-PL-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (7)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Porcine IL-12 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is 0.05‑0.15 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived porcine IL-12 protein

Porcine IL-12 p40
(Ile23 - Asn324)
Accession # Q28938

Porcine IL-12 p35
(Arg26 - Ser222)
Accession # Q29053

N-terminus

C-terminus

Accession #

Q28938 (p40) & Q29053 (p35)

N-terminal Sequence
Analysis

Ile23 (p40) & Arg26 (p35)

Structure / Form

Disulfide-linked heterodimer

SDS-PAGE

60-70 kDa, non-reducing conditions

Product Datasheets

912-PL (with carrier)

Product Datasheet

COA

SDS

912-PL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

912-PL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

912-PL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Porcine IL-12 subunits p35 and p40 share about 85% homology to the human subunits, but differ by containing a 3 aa addition in the p35 subunit and a 4 aa deletion in the p40 subunit. Porcine IL-12 induces proliferation of human lymphoblasts and IFN-gamma secretion by human and porcine lymph nodes. Porcine IL-12 has been detected in lymphoid tissues including inguinal and mesenteric lymph nodes, Peyer's patches, spleen and thymus.

References

Foss, D. et al. (1997) Vet. Immunol. Immunopathol. 57:121.

Long Name

Interleukin 12

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Alternate Names

CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Related Research Areas

Citations for Recombinant Porcine IL-12 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation
Authors: B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann
Microorganisms, 2021-08-28;9(9):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay
Protective porcine influenza virus-specific monoclonal antibodies recognize similar haemagglutinin epitopes as humans
Authors: B Holzer, P Rijal, A McNee, B Paudyal, V Martini, B Clark, T Manjegowda, FJ Salguero, E Bessell, JC Schwartz, K Moffat, M Pedrera, SP Graham, A Noble, M Bonnet-Di, RM La Ragione, W Mwangi, P Beverley, JW McCauley, RS Daniels, JA Hammond, AR Townsend, E Tchilian
PloS Pathogens, 2021-03-04;17(3):e1009330.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay
Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
Infect. Immun., 2017-03-23;85(4):.
Species: Porcine
Sample Types: Serum
Applications: ELISA (Standard)
Porcine CD8alphadim/-NKp46high NK cells are in a highly activated state.
Authors: Mair K, Mullebner A, Essler S, Duvigneau J, Storset A, Saalmuller A, Gerner W
Vet Res, 2013-03-01;44(0):13.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay
NKp46 expression discriminates porcine NK cells with different functional properties.
Authors: Mair KH, Essler SE, Patzl M, Storset AK, Saalmuller A, Gerner W
Eur. J. Immunol., 2012-05-01;42(5):1261-71.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay
Activation of Porcine Natural Killer (NK) Cells and Lysis of Foot-and-Mouth Disease Virus (FMDV) Infected Cells.
Authors: Toka FN, Nfon CK, Dawson H, Mark Estes D, Golde WT
J. Interferon Cytokine Res., 2009-03-01;0(0):.
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay
Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
J. Virol., 2006-01-01;80(1):372-82.
Applications: ELISA (Standard)