Recombinant Rat DLL1 Fc Chimera Protein, CF Summary
Product Specifications
| Rat DLL-1 (Ser22 - Ser534) Accession # P97677 |
IEGRMDP | Mouse IgG2A (Glu98 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3970-DL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: DLL1
Delta-like protein 1 (DLL1) is a 90 ‑ 100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF‑like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17‑ mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2 ‑ 4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7 ‑ 13). The up‑regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15 ‑ 16).
- Bettenhausen, B. et al. (1995) Development 121:2407.
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- Karanu, F.N. et al. (2001) Blood 97:1960.
- Sun, D. et al. (2008) J. Cell Sci. 121:3815.
- Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
- Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
- Takahashi, Y. et al. (2003) Development 130:4259.
- Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
- Kiernan, A.E. et al. (2005) Development 132:4353.
- Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
- Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
- Shimojo, H. et al. (2008) Neuron 58:52.
- Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
- Limbourg, A. et al. (2007) Circ. Res. 100:363.
- Purow, B.W. et al. (2005) Cancer Res. 65:2353.
- Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Citation for Recombinant Rat DLL1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Delta/Notch-Like EGF-Related Receptor (DNER) Is Not a Notch Ligand
PLoS ONE, 2016-09-13;11(9):e0161157.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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fibroblast derived ipsc lines were grown for 48 hr on gelatine-coated plates which contain 100 ng/ml rDLL1. mRNA level of Hes1 was detected to check if DLL1 stimulated Hes1 expression.
