Recombinant Rat EGF Protein Summary
Product Specifications
Asn974-Arg1026, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3214-EG
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3214-EG/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: EGF
Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
- Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
- Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
- Saggi, S.J. et al. (1992) DNA Cell Biol. 11:481.
- Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
- Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
- Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
- Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
- Qian, X. et al. (1999) J. Biol. Chem. 274:574.
Citations for Recombinant Rat EGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats
Authors: Z Berkova, K Zacharovov, A Patikova, I Leontovyc, Z Hladikova, D Cerveny, E Tihlarikov, V Nedela, P Girman, D Jirak, F Saudek
Journal of functional biomaterials, 2022-09-30;13(4):.
Species: Rat
Sample Types: Whole Cell
Applications: Cell Culture -
Sox9 is involved in the thyroid differentiation program and is regulated by crosstalk between TSH, TGFbeta and thyroid transcription factors
Authors: A López-Márq, C Carrasco-L, A Martínez-C, P Lemoine, CE Pierreux, P Santisteba
Scientific Reports, 2022-02-09;12(1):2144.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Let-7, Lin28 and Hmga2 Expression in Ciliary Epithelium and Retinal Progenitor Cells
Authors: LT Frasson, B Dalmaso, PS Akamine, ET Kimura, DE Hamassaki, CB Del Debbio
Investigative Ophthalmology & Visual Science, 2021-03-01;62(3):31.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Long noncoding RNA mediates stroke-induced neurogenesis
Authors: B Fan, W Pan, X Wang, M Wei, A He, A Zhao, M Chopp, ZG Zhang, XS Liu
Stem Cells, 2020-05-02;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells
Authors: M Nakamura, H Nishida, K Yoshizaki, H Akiyoshi, S Hatoya, K Sugiura, T Inaba
J. Vet. Med. Sci., 2020-04-06;0(0):.
Species: Rat
Sample Types: Cells
Applications: Cell Culture -
Implantation of engineered conduction tissue in the rat heart
Authors: W Zhang, X Li, S Sun, X Zhang
Mol Med Rep, 2019-02-05;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles
Authors: D Stöckmann, T Spannbruck, N Ale-Agha, P Jakobs, C Goy, N Dyballa-Ru, T Hornstein, A Kümper, A Kraegeloh, J Haendeler, K Unfried
Nanomaterials (Basel), 2018-04-23;8(4):.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Na�ve Schwann Cells in the Sciatic Nerve Injury Model
Authors: M Zhang, MH Jiang, DW Kim, W Ahn, E Chung, Y Son, G Chi
Biomed Res Int, 2017-06-20;2017(0):1252851.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Bioassay -
Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling
Authors: O Stojadinov, TC Wikramanay, AC Villasante, NC Yin, L Liang, E Hinde, J Escandon, M Tomic-Cani, DM Ansell, R Paus, JJ Jimenez
Heliyon, 2017-05-30;3(5):e00309.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
TGF-beta signalling in the adult neurogenic niche promotes stem cell quiescence as well as generation of new neurons.
Authors: Kandasamy M, Lehner B, Kraus S, Sander P, Marschallinger J, Rivera F, Trumbach D, Ueberham U, Reitsamer H, Strauss O, Bogdahn U, Couillard-Despres S, Aigner L
J Cell Mol Med, 2014-04-30;18(7):1444-59.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
EMBO J, 2014-02-20;33(7):702-18.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor.
Authors: Gilbert RE, Huang Q, Thai K, Advani SL, Lee K, Yuen DA, Connelly KA, Advani A
Kidney Int., 2011-03-09;79(12):1312-21.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
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