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Recombinant Rat Fc gamma RI/CD64 Protein, CF

Catalog #: 9558-FC Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9558-FC-100
Recombinant Rat Fc gamma RI/CD64 Protein Binding Activity
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Recombinant Rat Fc gamma RI/CD64 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Fc gamma  RI/CD64 is immobilized at 1 μg/mL, 100 μL/well, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.075-0.35 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Fc gamma RI/CD64 protein
Gln16-Pro285, with a C-terminal 6-His tag
Accession #
NP_001094306
N-terminal Sequence
Analysis
Thr21 observed & Gln16 inferred from enzymatic pyroglutamate treatment revealing Val17
Predicted Molecular Mass
31 kDa
SDS-PAGE
36-48 kDa, reducing conditions

Product Datasheets

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9558-FC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9558-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Rat Fc gamma RI/CD64 Protein Binding Activity View Larger

When Recombinant Rat Fc gamma RI/CD64 (Catalog # 9558‑FC) is immobilized at 1 μg/mL, 100 μL/well, Biotinylated Rat IgG binds with an ED50 of 0.075-0.35 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma  Rs, based on close relationships in their extracellular domains: Fc gamma  RI/CD64, Fc gamma RII/CD32, and Fc gamma  RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). The CD64 proteins are high affinity receptors (~10-8-10-9 M) that are capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) (4, 5). The high affinity of IgG by Fc gamma  RI permits the triggering of effector responses at low IgG concentrations typical of early immune response (6). The mature protein from this family consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fcɣ RI shares 78% and 64% amino acid sequence identity with mouse and human Fc gamma RI, respectively. Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils. Its expression is up-regulated during bacterial infections and sepsis (1, 5).

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Chenoweth, A.M et al. (2015) Immunol. Rev. 268:175.
  5. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  6. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
Long Name
Fc gamma Receptor I
Entrez Gene IDs
2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
Alternate Names
CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I

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