Recombinant Rat IL-1ra/IL-1F3 Protein Summary
Product Specifications
His27-Gln178, with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1545-RA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1545-RA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-1ra/IL-1F3
Interleukin 1 alpha (IL-1 alpha ), IL-1 beta, and IL-1 receptor antagonist (IL-1ra) are members of the IL-1 family. IL-1 alpha and IL-1 beta are agonists that bind the functional IL-1 receptor I (IL-1 RI), which then recruits the IL-1 receptor accessory protein (IL-1 R AcP/IL-1 R3) and initiates signal transduction pathways that include the NF-kappa B. JNK/AP-1, and p38 MAP kinase pathways. Both IL-1 alpha and IL-1 beta also bind the non‑signaling IL-1 RII that functions as a decoy receptor. IL-1ra is a receptor antagonist that binds both IL-1 RI and II with equal affinity as the IL-1 alpha and beta. Binding of IL-1ra to IL-1 RI does not initiate signal transduction pathways. IL-1 alpha and beta are pro‑inflammatory cytokines that play an important role in host defense. The imbalance between IL-1 ( alpha and beta ) and IL-1ra can influence the development of various pathologic conditions associated with chronic inflammation and tissue destruction. Pre‑clinical and clinical studies have indicated that IL-1ra may have therapeutic potential for the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia (1, 2).
Rat IL-1ra cDNA encodes a 178 amino acid (aa) residues secreted protein with a 26 aa signal peptide. Although intracellular isoforms of human IL-1ra that are transcribed from the same gene using different promotors have been described, analogous rat isoforms have not been reported. Rat IL-1ra shares 89% and 73% aa sequence homology with mouse and human IL-1ra, respectively. Secreted IL-1ra has been shown to be expressed by monocytes, macrophages, neutrophils, fibroblasts and hepatocytes (1, 2).
- Dinarello, C.A. (1996) Blood 87:2095 (review).
- Arend, W.P. (2002) Growth Factor Reviews 23:323 (review).
Citations for Recombinant Rat IL-1ra/IL-1F3 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Targeting the ?2-integrin LFA-1, reduces adverse neuroimmune actions in neuropathic susceptibility caused by prenatal alcohol exposure
Authors: JJ Sanchez, JE Sanchez, S Noor, CD Ruffaner-H, S Davies, CR Wagner, LL Jantzie, N Mellios, DD Savage, ED Milligan
Acta Neuropathol Commun, 2019-04-08;7(1):54.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms
Authors: L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo
J Neuroinflammation, 2018-02-08;15(1):36.
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay -
Possible protective effect of membrane lipid rafts against interleukin-1beta-mediated anti-proliferative effect in INS-1 cells.
Authors: Chentouf M, Guzman C, Hamze M, Gross R, Lajoix A, Peraldi-Roux S
PLoS ONE, 2014-07-28;9(7):e102889.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
A leptin-mediated central mechanism in analgesia-enhanced opioid reward in rats.
Authors: Lim G, Kim H, McCabe M, Chou C, Wang S, Chen L, Marota J, Blood A, Breiter H, Mao J
J Neurosci, 2014-07-16;34(29):9779-88.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats.
Authors: Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y
PLoS ONE, 2014-03-12;9(3):e90950.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
J. Neurochem., 2011-02-09;117(1):164-74.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Lysophosphatidic acid signals through multiple receptors in osteoclasts to elevate cytosolic calcium concentration, evoke retraction, and promote cell survival.
Authors: Lapierre DM, Tanabe N, Pereverzev A
J. Biol. Chem., 2010-06-15;285(33):25792-801.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay -
Reductions of acetylcholine release and nerve growth factor expression are correlated with memory impairment induced by interleukin-1beta administrations: effects of omega-3 fatty acid EPA treatment.
Authors: Taepavarapruk P, Song C
J. Neurochem., 2009-12-03;112(4):1054-64.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Antagonism of peripheral inflammation reduces the severity of status epilepticus.
Authors: Marchi N, Fan Q, Ghosh C, Fazio V, Bertolini F, Betto G, Batra A, Carlton E, Najm I, Granata T, Janigro D
Neurobiol. Dis., 2008-10-28;33(2):171-81.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress.
Authors: Koo JW, Duman RS
Proc. Natl. Acad. Sci. U.S.A., 2008-01-04;105(2):751-6.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
Interleukin-1 beta contributes to the upregulation of kappa opioid receptor mrna in dorsal root ganglia in response to peripheral inflammation.
Authors: Puehler W, Rittner HL, Mousa SA, Brack A, Krause H, Stein C, Schafer M
Neuroscience, 2006-06-19;141(2):989-98.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
The effects of endogenous interleukin-1 bioactivity on locus coeruleus neurons in response to bacterial and viral substances.
Authors: Borsody MK, Weiss JM
Brain Res., 2004-05-08;1007(1):39-56.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
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