Recombinant Rat Osteopontin (OPN) Protein, CF

Catalog #: 6359-OP
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
6359-OP-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Osteopontin (OPN) Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

When 1 x 10⁵ cells/well are added to Recombinant Rat Osteopontin/OPN coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour incubation at 37 °C. The ED₅₀ for this effect is 0.2‑1 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived rat Osteopontin/OPN protein
Met1-Asn317, with a C-terminal 6-His tag

Accession #

P08721

N-terminal Sequence
Analysis

Leu17

Predicted Molecular Mass

34.1 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Product Datasheets

6359-OP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6359-OP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Osteopontin/OPN

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta-1 (early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin-binding ligand N-linked glycoprotein) family of non-collagenous matricellular proteins (1 ‑ 3). Rat OPN is synthesized as a 317 amino acid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature rat OPN shares 79%, 62% and 50% aa sequence identity with mouse, human and bovine OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1 ‑ 4). Depending on tissue-specific modification by O- and N-glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45 ‑ 75 kDa (5, 6). The central region of OPN contains RGD and non-RGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury (6 ‑ 8). OPN can also be cleaved by MMP-3, -7, -9, and -12 within the SLAYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).

References

Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
Oldberg, A. et al. (1986) Proc. Natl. Acad. Sci. USA 83:8819.
Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
Takafuji, V. et al. (2007) Oncogene 26:6361.
Kiefer, F.W. et al. (2010) Diabetes 59:935.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Entrez Gene IDs

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Alternate Names

BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

Related Research Areas

Citations for Recombinant Rat Osteopontin (OPN) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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