Recombinant Rat OX40 Ligand/TNFSF4 Protein

Recombinant Rat OX40 Ligand/TNFSF4 Protein Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L; also known as TNFSF4, CD252, and gp34) is a type II transmembrane glycoprotein belonging to the TNF superfamily (1-3). OX40 Ligand coordinates with other costimulators (B7, CD40 Ligand/TNFSF5, CD30 Ligand/TNFSF8, CD27 Ligand/TNFSF7, and 4-1BB Ligand/TNFSF9) to manage the activation of the immune response. The rat OX40 Ligand cDNA encodes a 199 amino acid (aa) polypeptide with a short amino-terminal cytoplasmic domain and a 151 aa carboxy-terminal extracellular domain (ECD). The rat OX40 Ligand ECD shares 79% and 40% aa sequence identity with mouse and human OX40 Ligand ECD, respectively. OX40 Ligand is expressed on the surface of activated antigen presenting cells, B cells, T cells, and endothelial cells at sites of inflammation (4-6). Similar to other TNF superfamily members, membrane-bound OX40 Ligand is expressed as a homotrimer (7). OX40 Ligand binds to the type 1 transmembrane receptor, OX40, which is expressed on activated T cells. OX40 Ligand-OX40 signaling results in increased T cell survival, proliferation, and cytokine production (8, 9). It also modulates natural killer cell function, inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs), and promotes the maintenance of and recall response in memory T cells (7-13). OX40 Ligand-OX40 signaling is involved in allergic airway inflammation, graft-versus-host disease, and other autoimmune diseases (14-16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (13, 17).

Related Research Areas

• B Cell Activation
• Co-stimulatory and Co-inhibitory Molecules
• Co-stimulatory Molecules Associated with Tfh Cells
• Regulation of Apoptosis by TNF Superfamily Members
• Regulation of T Cell Co-stimulation by TNF Superfamily Members
• Regulatory T Cells (Tregs)
• TNF Superfamily
• TNF Superfamily Ligands