Sheep IgG Horseradish Peroxidase-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Sheep IgG Primary Antibody by Western Blot Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1 Antigen Affinity-purified Polyclonal Antibody (AF7565) using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Sheep IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody (AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of AIF in Human Kidney Detection of AIF in human kidney using 3 ug/mL of Sheep anti-human AIF primary antibody (AF5824) followed by 30 min incubation with Donkey anti Sheep HRP conjugated secondary antibodies (Catalog # HAF016) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Reconstitution Calculator
Preparation and Storage
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Sheep IgG Horseradish Peroxidase-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
Cell Reports, 2023-02-16;42(2):112133.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
Nature Communications, 2022-11-25;13(1):7269.
Species: Human, Mouse
Sample Types: Tissue Homogeantes, Tissue Homogenates
Applications: Western Blot -
alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth
Authors: S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland
JCI Insight, 2022-11-08;7(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells
Authors: G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi
International Journal of Molecular Sciences, 2022-10-22;23(21):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Aquilaria crassna Leaf Extract Ameliorates Glucose-Induced Neurotoxicity In Vitro and Improves Lifespan in Caenorhabditis elegans
Authors: N Pattaracho, N Sornkaew, W Warayanon, P Rangsinth, C Sillapacha, W Vongthip, S Chuchawank, A Prasansukl, T Tencomnao
Nutrients, 2022-09-05;14(17):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
Nature Immunology, 2022-07-06;23(7):1021-1030.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation
Authors: L Zhou, W Zhou, AM Joseph, C Chu, GG Putzel, B Fang, F Teng, M Lyu, H Yano, KI Andreasson, E Mekada, G Eberl, GF Sonnenberg
Nature Immunology, 2022-01-31;23(2):251-261.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage
Authors: SM Præstholm, CM Correia, VE Goitea, MS Siersbæk, M Jørgensen, JF Havelund, TÅ Pedersen, NJ Færgeman, L Grøntved
Cell Reports, 2021-11-02;37(5):109938.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting shear gradient activated von Willebrand factor by the novel single-chain antibody A1 reduces occlusive thrombus formation in vitro
Authors: T Hoefer, A Rana, B Niego, S Jagdale, HJ Albers, EE Gardiner, RK Andrews, AD Van der Me, CE Hagemeyer, E Westein
Haematologica, 2021-11-01;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains
Authors: PR Elliott, D Leske, J Wagstaff, L Schlicher, G Berridge, S Maslen, F Timmermann, B Ma, R Fischer, SMV Freund, D Komander, M Gyrd-Hanse
Cell Reports, 2021-10-05;37(1):109777.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays
Authors: MS Szymczak, WK Reeves, MM Miller
Viruses, 2021-08-30;13(9):.
Species: Cercopithecus aethiops
Sample Types: Cell Lysates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021-04-01;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth
Authors: X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee
JCI Insight, 2020-10-02;5(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Silencing of Wwox Increases Nuclear Import of Dvl proteins in Head and Neck Cancer
Authors: A Celebi, C Orhan, B Seyhan, N Buyru
J Cancer, 2020-04-06;11(14):4030-4036.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular Protease ADAMTS1 Is Required at Early Stages of Human Uveal Melanoma Development by Inducing Stemness and Endothelial-Like Features on Tumor Cells
Authors: C Peris-Torr, MDC Plaza-Calo, R López-Domí, S Domínguez-, A Barrientos, P Carmona-Sá, JC Rodríguez-
Cancers (Basel), 2020-03-27;12(4):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
DNA methylation?regulated miR?155?5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10
Authors: W Luo, H Zhang, X Liang, R Xia, H Deng, Q Yi, L Lv, L Qian
Oncol. Rep., 2020-03-06;43(5):1692-1704.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
Authors: C Piro-Mégy, E Sarzi, A Tarrés-Sol, M Péquignot, F Hensen, M Quilès, G Manes, A Chakrabort, A Sénéchal, B Bocquet, C Cazevieill, A Roubertie, A Müller, M Charif, D Goudenège, G Lenaers, H Wilhelm, U Kellner, N Weisschuh, B Wissinger, X Zanlonghi, C Hamel, JN Spelbrink, M Solà, C Delettre
J. Clin. Invest., 2020-01-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Preclinical investigation of potential use of thymidine phosphorylase-targeting tracer for diagnosis of nonalcoholic steatohepatitis
Authors: K Higashikaw, S Horiguchi, M Tarisawa, Y Shibata, K Ohkura, H Yasui, H Takeda, Y Kuge
Nucl. Med. Biol., 2019-12-16;82(0):25-32.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Homozygous NLRP1 gain-of-function mutation in siblings with a syndromic form of recurrent respiratory papillomatosis
Authors: SB Drutman, F Haerynck, FL Zhong, D Hum, NJ Hernandez, S Belkaya, F Rapaport, SJ de Jong, D Creytens, SJ Tavernier, K Bonte, S De Scheppe, J van der We, L Lorenzo-Di, A Wullaert, X Bossuyt, G Orth, VR Bonagura, V Béziat, L Abel, E Jouanguy, B Reversade, J Laurent-Ca
Proc. Natl. Acad. Sci. U.S.A., 2019-09-04;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Natural single-nucleotide deletion in chymotrypsinogen C gene increases severity of secretagogue-induced pancreatitis in C57BL/6 mice
Authors: A Geisz, Z Jancsó, BC Németh, E Hegyi, M Sahin-Tóth
JCI Insight, 2019-06-18;5(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Pretreatment of Populus tomentiglandulosa protects hippocampal CA1 pyramidal neurons from ischemia-reperfusion injury in gerbils via increasing SODs expressions and maintaining BDNF and IGF-I expressions
Authors: TK Lee, JH Park, JH Ahn, H Kim, M Song, JC Lee, JD Kim, YH Jeon, JH Choi, CH Lee, IK Hwang, BC Yan, MH Won, IJ Kang
Chin J Nat Med, 2019-06-01;17(6):424-434.
Species: Gerbil
Sample Types: Tissue Homogenates
Applications: Western Blot -
Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer
Authors: TP Sandberg, MPME Stuart, J Oosting, RAEM Tollenaar, CFM Sier, WE Mesker
BMC Cancer, 2019-03-29;19(1):284.
Species: Human
Sample Types: Whole Tissue
Applications: Western Blot -
Association of cardiac myosin binding protein-C with the ryanodine receptor channel: putative retrograde regulation?
Authors: PJ Stanczyk, M Seidel, J White, C Viero, CH George, S Zissimopou, FA Lai
J. Cell. Sci., 2018-08-03;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
Authors: JM Van Kampen, DG Kay
PLoS ONE, 2017-08-24;12(8):e0182896.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
UBASH3A Mediates Risk for Type 1 Diabetes through Inhibition of T-Cell Receptor-Induced NF-?B Signaling
Authors: Y Ge, TK Paisie, JRB Newman, LM McIntyre, P Concannon
Diabetes, 2017-06-12;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Polymerization-Incompetent Uromodulin in the Pregnant Stroke-Prone Spontaneously Hypertensive Rat
Authors: S Mary, HY Small, J Siwy, W Mullen, A Giri, C Delles
Hypertension, 2017-03-27;69(5):910-918.
Species: Rat
Sample Types: Protein
Applications: Western Blot -
Targeting tachykinin receptors in neuroblastoma
Oncotarget, 2017-01-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Rnd3-induced cell rounding requires interaction with Plexin-B2
J Cell Sci, 2016-09-21;0(0):.
Species: Primate - Chlorocebus sabaeus (Green Monkey)
Sample Types: Cell Lysates
Applications: Western Blot -
Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
J Neurosci, 2016-08-31;36(35):9201-16.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Sprouting angiogenesis is regulated by shedding of the C-type lectin family 14, member A (CLEC14A) ectodomain, catalyzed by rhomboid-like 2 protein (RHBDL2).
Authors: Noy P, Swain R, Khan K, Lodhia P, Bicknell R
FASEB J, 2016-03-03;30(6):2311-23.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis.
Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S
PLoS ONE, 2015-08-25;10(8):e0136563.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment.
Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A
PLoS Genet, 2015-07-21;11(7):e1005386.
Species: Human
Sample Types: Cell Lysates
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Blocking CLEC14A-MMRN2 binding inhibits sprouting angiogenesis and tumour growth.
Authors: Noy P, Lodhia P, Khan K, Zhuang X, Ward D, Verissimo A, Bacon A, Bicknell R
Oncogene, 2015-03-09;34(47):5821-31.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies.
Authors: Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprussel A, Lindner S, De Preter K, Florin A, Heukamp L, Klein-Hitpass L, Astrahantseff K, Kumps C, Speleman F, Eggert A, Westermann F, Schramm A, Schulte J
Oncogene, 2014-09-01;34(26):3357-68.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
ADAMTS1 inhibits lymphangiogenesis by attenuating phosphorylation of the lymphatic endothelial cell-specific VEGF receptor.
Authors: Inagaki J, Takahashi K, Ogawa H, Asano K, Faruk Hatipoglu O, Cilek M, Obika M, Ohtsuki T, Hofmann M, Kusachi S, Ninomiya Y, Hirohata S
Exp Cell Res, 2014-03-11;323(2):263-75.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors.
Authors: St Jean A, Swofford C, Panteli J, Brentzel Z, Forbes N
Mol Ther, 2014-03-04;22(7):1266-74.
Species: Bacteria - E. Coli, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Antibodies that detect O-linked beta-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins.
Authors: Tashima Y, Stanley P
J Biol Chem, 2014-02-26;289(16):11132-42.
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SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/beta-catenin signaling.
Authors: Thu K, Radulovich N, Becker-Santos D, Pikor L, Pusic A, Lockwood W, Lam W, Tsao M
Oncogene, 2013-01-14;33(3):279-88.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription.
Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A
PLoS ONE, 2012-04-12;7(4):e34703.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions.
Authors: Nakamura R, Nakamoto C, Obama H
J. Biol. Chem., 2011-12-08;287(5):3282-91.
Species: Ovine
Sample Types: Antibody
Applications: Western Blot -
Neurocan contributes to the molecular heterogeneity of the perinodal ECM.
Authors: Bekku Y, Oohashi T
Arch. Histol. Cytol., 2010-01-01;73(2):95-102.
Species: Mouse
Sample Types: Tissue Homogenates
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Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
J. Pathol., 2009-10-01;219(2):173-81.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Secondary Antibody
FAQs
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Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
- All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
Reviews for Sheep IgG Horseradish Peroxidase-conjugated Antibody
Average Rating: 4.9 (Based on 12 Reviews)
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Dilution: 1:1000 in skim milk in TBST (Incubation: 1hr at room temperature)
Western blotting detection of CD47 using sheep polyclonal antibody (AF4670).
Primary Ab = Anti SSBP1 Polyclonal Sheep IgG AF6588 (1:200)
Secondary Ab = 1:1000
dil: 1:6000 in 5% skim milk powder and TBST.