Simple Plex Human alpha 2-Macroglobulin Cartridge

Catalog #: SPCKB-PS-008932 Datasheet / COA / SDS

Simple Plex Human alpha 2-Macroglobulin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-00893
SPCKC-PS-008932
SPCKA-PS-00893

Control Products Available

897116: Simple Plex Control for Human alpha 2-Macroglobulin

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All alpha 2-Macroglobulin Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human alpha 2-Macroglobulin Cartridge Summary

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

16.97 pg/mL

Assay Range

81.9 - 125,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid)

Specificity

Natural and recombinant human alpha 2-Macroglobulin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human alpha 2-Macroglobulin ( alpha 2-Macroglobulin) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay)

Inter-Assay Precision (Precision between assays)

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	64	68
Mean (pg/mL)	515	40826	766	38297
Standard Deviation	29.7	3940	85.8	4381
CV%	5.8	9.7	11.2	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	101	98-104

Linearity

Samples containing and/or spiked with high concentrations of human alpha 2-Macroglobulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human alpha 2-Macroglobulin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha 2-Macroglobulin

Alpha 2-Macroglobulin (A2M) is an abundant plasma glycoprotein that has structural similarity to other A2M family members including complement components C3, C4 and C5. A2M is a tetramer (720-kDa) of 4 identical subunits (180-kDa) formed by two disulfide bond-linked dimers. It is a general and irreversible protease inhibitor capable of inhibiting all four classes of proteases by a unique trapping mechanism. A2M also binds many cytokines and growth factors. In the brain, A2M binds soluble beta Amyloid. A2M is recognized as a late onset Alzheimers disease gene.

Entrez Gene IDs:

2 (Human)

Alternate Names:

A2M; alpha 2Macroglobulin; alpha 2-Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7