Simple Plex Human Angiopoietin-like Protein 4 Cartridge

Catalog #: SPCKB-PS-000291 Datasheet / COA / SDS

Simple Plex Human Angiopoietin-like Protein 4/ANGPTL4 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000291
SPCKA-PS-000291
SPCKC-PS-000291

Control Products Available

898432: Simple Plex Control for Human Angiopoietin-like Protein 4

Human Angiopoietin-like 4 Simple Plex Assay

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All Angiopoietin-like Protein 4/ANGPTL4 Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Angiopoietin-like Protein 4 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

93.2 pg/mL

Assay Range

546.0 - 333,200 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Angiopoietin-like 4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Angiopoietin-like 4 in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	18
Mean (pg/mL)	4324	190343	5327	222216
Standard Deviation	293	17170	795	29685
CV%	5	9	14.9	13.4

Recovery

Recovery was not necessary as Angiopoietin-like 4 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Angiopoietin-like 4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human Angiopoietin-like 4 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:

51129 (Human); 57875 (Mouse); 362850 (Rat)

Alternate Names:

Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein