Simple Plex Human BCMA/TNFRSF17 Cartridge

Catalog #: SPCKB-PS-000768 Datasheet / COA / SDS

Simple Plex Human BCMA/TNFRSF17 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000768
SPCKA-PS-000768
SPCKC-PS-000768

Control Products Available

898628: Simple Plex Control for Human BCMA/TNFRSF17

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human BCMA/TNFRSF17 Cartridge Summary

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.54 pg/mL

Assay Range

2.6 - 10,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human BCMA.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human B Cell Maturation Antigen/TNFRSF17 (BCMA) in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	35	35
Mean (pg/mL)	58.8	2931	57.7	2971
Standard Deviation	2.96	170	6.4	283
CV%	5	5.8	11.1	9.5

Recovery

Recovery was not necessary as BCMA shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human BCMA were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human BCMA Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Long Name:

B Cell Maturation Factor

Entrez Gene IDs:

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Alternate Names:

B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17