Simple Plex Human CCL8/MCP-2 Cartridge

Catalog #: SPCKB-PS-000478 Datasheet / COA / SDS

Simple Plex Human CCL8/MCP-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000478
SPCKA-PS-000478
SPCKC-PS-000478

Control Products Available

898538: Simple Plex Control for Human CCL8/MCP-2

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CCL8/MCP-2 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

0.05 pg/mL

Assay Range

0.2 - 933 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human MCP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human MCP-2 in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	33	33
Mean (pg/mL)	5.15	201	5.3	227
Standard Deviation	0.266	16.6	0.598	16.5
CV%	5.2	8.3	11.3	7.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	97	93-102
Heparin Plasma (n=4)	97	86-103
Serum (n=4)	101	92-107

Linearity

Samples containing and/or spiked with high concentrations of human MCP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human MCP-2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL8/MCP-2

MCP-2 is a CC chemokine subfamily monocyte chemotactic protein. MCP-2 also chemoattracts activated NK cells, CD4+ and CD8+ T lymphocytes, and eosinophils and can induce histamine secretion from basophils. MCP-2 shares 62% amino acid sequence identity with MCP-1 and 58% with MCP-3.

Entrez Gene IDs:

6355 (Human); 20307 (Mouse)

Alternate Names:

C-C motif chemokine 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8