Simple Plex Human CD27/TNFRSF7 Cartridge

Catalog #: SPCKB-PS-000639 Datasheet / COA / SDS
Simple Plex Human CD27/TNFRSF7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000639
SPCKA-PS-000639
SPCKC-PS-000639
Control Products Available
Human CD27/TNFRSF7 Ella Assay
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Simple Plex Human CD27/TNFRSF7 Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (13 uL)
Sensitivity
30.7 pg/mL
Assay Range
30.7 - 46,900 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)
Specificity
Natural and recombinant human CD27.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CD27 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 15 15
Mean (pg/mL) 346 14787 310 14320
Standard Deviation 15.2 1440 20.7 960
CV% 4.4 9.7 6.7 6.7

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 102 94-117

Linearity

Samples containing and/or spiked with high concentrations of human CD27 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human CD27/TNFRSF7 Ella Assay Linearity

Scientific Data

Human CD27/TNFRSF7 Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.

Entrez Gene IDs:
939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
Alternate Names:
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

FAQs

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