Simple Plex Human FABP2/I-FABP Cartridge

Catalog #: SPCKB-PS-000361 Datasheet / COA / SDS

Simple Plex Human FABP2/I-FABP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000361
SPCKA-PS-000361
SPCKC-PS-000361

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898181: Simple Plex Control for Human FABP2/I-FABP

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human FABP2/I-FABP Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)

Sensitivity

5.66 pg/mL

Assay Range

31.1 - 118,560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human FABP2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human FABP2 in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	47
Mean (pg/mL)	792	37335	777	36690
Standard Deviation	43	842	67	2714
CV%	5.4	2.3	8.6	7.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	102	95-109
Heparin Plasma (n=3)	102	100-106
Serum (n=4)	104	86-113

Linearity

Samples containing and/or spiked with high concentrations of human FABP2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human FABP2 Simple Plex Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name:

Fatty Acid-Binding Protein 2/Intestinal FABP

Entrez Gene IDs:

2169 (Human); 25598 (Rat)

Alternate Names:

FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein