Simple Plex Human Resistin Cartridge

Catalog #: SPCKB-PS-001017 Datasheet / COA / SDS

Simple Plex Human Resistin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-001017
SPCKA-PS-001017
SPCKC-PS-001017

Control Products Available

898784: Simple Plex Control for Human Resistin

3 Images

Product Details

Supplemental Products

Reviews

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Resistin Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 uL), EDTA Plasma (5 uL), Heparin Plasma (5 uL)

Sensitivity

0.35 pg/mL

Assay Range

6.6 - 25,150 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Resistin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Resistin in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	25	25
Mean (pg/mL)	137	7242	130	6913
Standard Deviation	13.5	460	14.4	644
CV%	9.9	6.4	11	9.3

Recovery

Recovery was not necessary as Resistin shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Resistin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

Human Resistin Simple Plex Assay Standard Curve

View Larger

Human Resistin Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Resistin

Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.

Entrez Gene IDs:

56729 (Human); 57264 (Mouse)

Alternate Names:

Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1