Simple Plex Human uPAR Cartridge

Catalog #: SPCKB-PS-007370 Datasheet / COA / SDS

Simple Plex Human uPAR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-007370
SPCKA-PS-007370
SPCKC-PS-007370

Control Products Available

897052: Simple Plex Control for Human uPAR

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All uPAR Simple Plex Assays

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human uPAR Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (10 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)

Sensitivity

0.2 pg/mL

Assay Range

1.7 - 16,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)

Specificity

Natural and recombinant human uPAR.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human UPAR in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine and cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	49	50
Mean (pg/mL)	120	6099	110	5748
Standard Deviation	6.8	261	8.51	558
CV%	5.7	4.3	7.7	9.7

Recovery

Recovery was not necessary at uPAR shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of uPAR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

N/A

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Human uPAR Ella Assay Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: uPAR

The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.

Long Name:

Urokinase-type Plasminogen Activator Receptor

Entrez Gene IDs:

5329 (Human); 18793 (Mouse); 102139334 (Cynomolgus Monkey)

Alternate Names:

CD87 antigen; CD87; Monocyte activation antigen Mo3; plasminogen activator, urokinase receptor; PLAUR; uPAR; U-PAR; UPARurokinase plasminogen activator surface receptor; u-plasminogen activator receptor form 2; URKRMO3