Simple Plex Mouse IL-7 Cartridge
Simple Plex Mouse IL-7 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||
---|---|---|---|---|
Sample | 1 | 2 | 1 | 2 |
n | 16 | 16 | 91 | 88 |
Mean (pg/mL) | 289 | 14906 | 202 | 9468 |
Standard Deviation | 13.1 | 567 | 19.8 | 953 |
CV% | 4.5 | 3.8 | 9.8 | 10.1 |
Recovery
Recovery at three different spiked concentrations within the range of the assay was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
EDTA Plasma (n=4) | 110 | 98-120 |
Heparin Plasma (n=4) | 117 | 115-120 |
Serum (n=4) | 113 | 109-116 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
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