Synapsin I Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named
A-E/F. Domains A-D lie between aa 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of Ser603 and 566 in domain D, and Ser9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.

Product Datasheets

Product Specific Notices

1. Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
2. Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
3. Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
4. McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
5. Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.

Related Research Areas

• Neuronal Markers
• Synaptic SNAREs