Thioflavin T
Chemical Name: 2-[4-(Dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
Purity: ≥95%
Biological Activity
Key information: Thioflavin T is a cell-permeable fluorescent amyloid stain.Used for: staining of insoluble senile plaques of Aβ in brain tissues, monitoring the kinetics of in vitro polyglutamine amyloid formation of tNhtt-42Q aggregates in a Huntington's disease cell model. Thioflavin T is used to confirm formation of β sheet structure from mutant huntingtin exon-1 aggregates (Ex1Q48) in vitro.
Application: fluorescence microscopy.
Properties and Photophysical Data: Thioflavin T is a cationic benzothiazole. It increases in fluorescence upon strong binding (Ki = 580 nM) to the stacked β sheets of amyloid fibrils. Excitation and emission maxima (λ) are 440 nm and 485 nm, respectively; quantum yield = 0.43; extinction coefficient = 31600 M-1cm-1.
This product is supplied with a high degree of hydration and some residual inorganic salts, the amount of which are batch dependent. Please refer to the Certificate of Analysis to obtain the batch specific Net Product Content.
Optical Data for Thioflavin T
| Emission Color | Blue |
|---|---|
| λabs | 440 nm |
| λem | 485 nm |
| Extinction Coefficient (ε) | 31600 M-1cm-1 |
| Quantum Yield (φ) | 0.43 |
| Cell Permeable | Yes |
| Application | Amyloid Stain |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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