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Total BDNF ELISA Kit - Quantikine

Catalog #: DBNT00
43 Citations 4 Reviews Datasheet / COA / SDS
Detects free and Trk-bound BDNF
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DBNT00
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PDBNT00
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QC218: Quantikine Immunoassay Control Set 1183 for Total BDNF
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Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve
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Product Details
Citations (43)
FAQs
Supplemental Products
Reviews (4)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Total BDNF Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor EDTA Plasma (10 uL), Heparin Plasma (50 uL), Platelet-poor Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity
1.35 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant free, trk-bound, pro-, and mature BDNF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine/Human milk - Samples from apparently healthy volunteers were evaluated for the presence of Total BDNF in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Human serum (n=36)35,6159042-57,8309790
Human PP EDTA plasma (n=36)30794.9-902197
Human PP heparin plasma (n=36)595100-2245491
Rat serum (n=10)52532554-69661438
Rat PP EDTA plasma (n=5)29487.5-416138
Rat PP heparin plasma (n=5)294103-713248

Sample TypeMean of Detectable (pg/mL)% DetectableRange (pg/mL)
Human Urine (n=20)ND0ND
Human milk (n=10)ND0ND
Mouse serum (n=10)55.450ND-147
Mouse PP EDTA plasma (n=5)21.460ND-29.7
Mouse PP heparin plasma (n=5)17.540ND-19.1
Mouse urine (n=5)ND0ND
Rat urine (n=5)ND0ND
Canine serum (n=4)ND0ND
Canine EDTA plasma (n=4)ND0ND
Canine heparin plasma (n=4)ND0ND
Porcine serum (n=4)ND0ND
Porcine EDTA plasma (n=4)ND0ND
Porcine heparin plasma (n=4)ND0ND
ND=Non-detectable

Cell Culture Supernates: 
Human peripheral blood leukocytes (1 x 106 cells) were cultured in RPMI supplemented with 10% fetal bovine serum. The cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of Total BDNF.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
Unstimulated25.521.2
Stimulated24.424.4

Human peripheral blood mononuclear cells (50 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate overnight. The cells were cultured unstimulated or stimulated with 1.0 μg/mL LPS for 1 day. Aliquots of the cell culture supernates were removed, assayed for levels of Total BDNF, and measured 2870 pg/mL and 2853 pg/mL, respectively.

MG-63 human osteosarcoma cells were cultured in DMEM supplemented with 5% fetal bovine serum and 4 mM L-glutamine and grown until confluent. An aliquot of the cell culture supernate was removed, assayed for Total BDNF, and measured 156 pg/mL.

Brain tissues from mice and rats were removed and rinsed in PBS and kept on ice. The tissue was homogenized using a tissue homogenizer and seeded into media containing RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated for 2 or 3 days. Aliquots of the cell culture supernates were removed and assayed for levels of Total BDNF.

Sample TypeObserved Levels (pg/mL)
Mouse brain (2 days)306
Rat brain (3 days) 196

Tissue lysates:
Human brain tissues were prepared in RIPA buffer with protease inhibitors. The working buffer was kept on ice and used within 2-3 days. Aliquots of the tissue lysates were removed and assayed for Total BDNF.

Sample Typepg/mg of Cell Lysate 
Human cerebellum515
Human hypothalamus441

Brains from mice were rinsed with PBS and homogenized with a tissue homogenizer in PBS. An equal volume of Lysis Buffer 17 was added and tissues were lysed at room temperature for 30 minutes with gentle agitation. Debris was then removed by centrifugation. An aliquot of each cell lysate was removed, assayed for levels of Total BDNF, and measured 1472 pg/mg of cell lysate.





Product Summary

The Quantikine Total BDNF Immunoassay is a 3.5 hour solid phase ELISA designed to measure BDNF in cell culture supernates, tissue lysates, serum, plasma, urine, and human milk. It contains recombinant human BDNF expressed in Sf 21 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant BDNF accurately. Results obtained measuring natural BDNF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural BDNF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 97.9 311 586 98.8 296 594
Standard Deviation 3.12 7.52 17.5 7.07 12.7 28
CV% 3.2 2.4 3 7.2 4.3 4.7

Recovery

The recovery of Total BDNF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 97-113
Cell Lysis Buffer (n=4) 94 87-100
Human Milk (n=4) 94 87-99
Platelet-poor EDTA Plasma (n=4) 95 76-110
Platelet-poor Heparin Plasma (n=4) 95 75-111
Urine (n=4) 93 75-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total BDNF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Linearity

Scientific Data

N/A Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve View Larger

Human/Mouse/Rat/Porcine/Canine Total BDNF ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Total BDNF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Circuit Training Improves the Levels of ?-Amyloid and Brain-Derived Neurotrophic Factor Related to Cognitive Impairment Risk Factors in Obese Elderly Korean Women
    Authors: Kang, D;Koh, S;Kim, T;Bressel, E;Kim, D;
    Journal of clinical medicine
    Species: Human
    Sample Types: Serum
  2. Vagus nerve stimulation (VNS) modulates synaptic plasticity in the rat infralimbic cortex via Trk-B receptor activation to reduce drug-seeking
    Authors: Driskill, CM;Childs, JE;Phensy, AJ;Rodriguez, SR;O'Brien, JT;Lindquist, KL;Naderi, A;Bordieanu, B;McGinty, JF;Kroener, S;
    bioRxiv : the preprint server for biology
    Species: Rat
    Sample Types: Tissue Homogenates
  3. Brain-derived neurotrophic factor contributes to neurogenesis after intracerebral hemorrhage: a rodent model and human study
    Authors: Lin, TC;Tsai, YC;Chen, YA;Young, TH;Wu, CC;Chiang, YH;Kao, CH;Huang, AP;Hsu, YH;Chen, KY;Tsai, LK;
    Frontiers in cellular neuroscience
    Species: Rat, Human
    Sample Types: Cerebrospinal Fluid
  4. Changes in cortisol awakening responses (CAR) in menopausal women through short-term marine healing retreat program with specific factors affecting each CAR index
    Authors: Y Moon, E Lee, S Lee, GI Son, H Byeon, HR Shin, B Bolortsets
    PLoS ONE, 2023-04-19;18(4):e0284627.
    Species: Human
    Sample Types: Serum
  5. Exercise-Induced Elevated BDNF Concentration Seems to Prevent Cognitive Impairment after Acute Exposure to Moderate Normobaric Hypoxia among Young Men
    Authors: M Chroboczek, S Kujach, M ?uszczyk, H Soya, R Laskowski
    International Journal of Environmental Research and Public Health, 2023-02-18;20(4):.
    Species: Human
    Sample Types: Serum
  6. Increasing Adiponectin Signaling by Sub-Chronic AdipoRon Treatment Elicits Antidepressant- and Anxiolytic-Like Effects Independent of Changes in Hippocampal Plasticity
    Authors: DA Formolo, TH Lee, J Yu, K Lin, G Chen, GS Kranz, SY Yau
    Biomedicines, 2023-01-18;11(2):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Changes in cortisol awakening responses (CAR) in menopausal women through short-term marine healing retreat program with specific factors affecting each CAR index
    Authors: Y Moon, E Lee, S Lee, GI Son, H Byeon, HR Shin, B Bolortsets
    PLoS ONE, 2023;18(4):e0284627.
    Species: Human
    Sample Types: Serum
  8. Dihydromyricetin ameliorates social isolation-induced anxiety by modulating mitochondrial function, antioxidant enzymes, and BDNF
    Authors: AJ Al Omran, S Watanabe, EC Hong, SG Skinner, M Zhang, J Zhang, XM Shao, J Liang
    Neurobiology of stress, 2022-10-30;21(0):100499.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Intravenous transplantation of amnion-derived mesenchymal stem cells promotes functional recovery and alleviates intestinal dysfunction after spinal cord injury
    Authors: S Takamiya, M Kawabori, K Yamazaki, S Yamaguchi, A Tanimori, K Yamamoto, S Ohnishi, T Seki, K Konno, KK Tha, D Hashimoto, M Watanabe, K Houkin, M Fujimura
    PLoS ONE, 2022-07-08;17(7):e0270606.
    Species: Rat
    Sample Types: Tissue Homogenates
  10. The Functional Vision Restorative Effect of Crocin via the BDNF-TrkB Pathway: An In Vivo Study
    Authors: JL Wu, SL Yang, YC Ho, CH Chen, BR Tasi, MC Lee, BY Chen
    Nutrients, 2022-04-20;14(9):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Thrombin Preconditioning Improves the Therapeutic Efficacy of Mesenchymal Stem Cells in Severe Intraventricular Hemorrhage Induced Neonatal Rats
    Authors: SY Jung, YE Kim, WS Park, SY Ahn, DK Sung, SI Sung, KM Joo, SG Kim, YS Chang
    International Journal of Molecular Sciences, 2022-04-18;23(8):.
    Species: Rat
    Sample Types: CSF
  12. Exploring the relationship between metal exposure, BDNF, and behavior in adolescent males
    Authors: A Rodríguez-, V Mustieles, SC D'Cruz, L Legoff, F Gil, P Olmedo, I Reina-Pére, A Mundo, M Molina, F Smagulova, A David, C Freire, MF Fernández
    Oncogene, 2021-10-28;239(0):113877.
    Species: Human
    Sample Types: Serum
  13. Dental pulp stem cells stimulate neuronal differentiation of PC12 cells
    Authors: N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven
    Neural regeneration research, 2021-09-01;16(9):1821-1828.
    Species: Rat
    Sample Types: Cell Culture Supernates
  14. Exercise-induced increases in Anandamide and BDNF during extinction consolidation contribute to reduced threat following reinstatement: Preliminary evidence from a randomized controlled trial
    Authors: KM Crombie, A Sartin-Tar, K Sellnow, R Ahrenholtz, S Lee, M Matalamaki, NE Almassi, CJ Hillard, KF Koltyn, TG Adams, JM Cisler
    Psychoneuroendocrinology, 2021-07-09;132(0):105355.
    Species: Human
    Sample Types: Serum
  15. Quercetin protects against diabetic retinopathy in rats by inducing heme oxygenase-1 expression
    Authors: GR Chai, S Liu, HW Yang, XL Chen
    Neural regeneration research, 2021-07-01;16(7):1344-1350.
    Species: Rat
    Sample Types: Cell Culture Supernates
  16. An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis
    Authors: R Li, C Ma, Y Xiong, H Zhao, Y Yang, L Xue, B Wang, T Xiao, J Chen, X Lei, B Ma, J Zhang
    Biomolecules, 2021-06-09;11(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. RAM-589.555 favors neuroprotective and anti-inflammatory profile of CNS-resident glial cells in acute relapse EAE affected mice
    Authors: R Zilkha-Fal, T Rachutin-Z, L Cleaver, M Gurevich, A Achiron
    J Neuroinflammation, 2020-10-21;17(1):313.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Mindfulness-Based Program Plus Amygdala and Insula Retraining (MAIR) for the Treatment of Women with Fibromyalgia: A Pilot Randomized Controlled Trial
    Authors: JP Sanabria-M, J Montero-Ma, A Feliu-Sole, V Gasión, M Navarro-Gi, H Morillo-Sa, A Colomer-Ca, X Borràs, M Tops, JV Luciano, J García-Cam
    J Clin Med, 2020-10-11;9(10):.
    Species: Human
    Sample Types: Serum
  19. Computational Analysis of Clinical and Molecular Markers and New Theranostic Possibilities in Primary Open-Angle Glaucoma
    Authors: MD Pinazo-Dur, JJ García-Med, JM Bolarín, SM Sanz-Gonzá, M Valero-Vel, J Abellán-Ab, V Zanón-More, J Moreno-Mon
    Journal of Clinical Medicine, 2020-09-21;9(9):.
    Species: Human
    Sample Types: Aqueous Humor
  20. Effect of football activity and physical fitness on information processing, inhibitory control and working memory in adolescents
    Authors: RA Williams, SB Cooper, KJ Dring, L Hatch, JG Morris, C Sunderland, ME Nevill
    BMC Public Health, 2020-09-14;20(1):1398.
    Species: Human
    Sample Types: Serum
  21. Long-term effects of experimental carotid stenosis on hippocampal infarct pathology, neurons and glia and amelioration by environmental enrichment
    Authors: W Stevenson, Y Hase, E Wilson, A Hollins, M Hase, A Ennaceur, L Craggs, M Ihara, K Horsburgh, RN Kalaria
    Brain Res. Bull., 2020-07-21;163(0):72-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Infusion of Plasma from Exercised Mice Ameliorates Cognitive Dysfunction by Increasing Hippocampal Neuroplasticity and Mitochondrial Functions in 3xTg-AD Mice
    Authors: TW Kim, SS Park, JY Park, HS Park
    Int J Mol Sci, 2020-05-06;21(9):.
    Species: Mouse
    Sample Types: Plasma
  23. Alpha-Lipoic Acid Ameliorates Radiation-Induced Salivary Gland Injury by Preserving Parasympathetic Innervation in Rats
    Authors: JH Kim, BK Jeong, SJ Jang, JW Yun, MH Jung, KM Kang, TG Kim, SH Woo
    Int J Mol Sci, 2020-03-25;21(7):.
    Species: Rat
    Sample Types: Serum
  24. Electroacupuncture Ameliorates Cerebral I/R-Induced Inflammation through DOR-BDNF/TrkB Pathway
    Authors: Y Geng, Y Chen, W Sun, Y Gu, Y Zhang, M Li, J Xie, X Tian
    Evid Based Complement Alternat Med, 2020-03-16;2020(0):3495836.
    Species: Rat
    Sample Types: Cell Culture Supernates
  25. Muscle Injury Induces Postoperative Cognitive Dysfunction
    Authors: L Guéniot, V Lepere, GF De Medeiro, A Danckaert, P Flamant, M Le Dudal, O Langeron, PL Goossens, F Chrétien, G Jouvion
    Sci Rep, 2020-02-17;10(1):2768.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Systematic Balance Exercises Influence Cortical Activation and Serum BDNF Levels in Older Adults
    Authors: J Kubica, J Szymura, A Domagalik, S Golda, M Wiecek, M Fafrowicz, T Marek, J Pera
    J Clin Med, 2019-11-07;8(11):.
    Species: Human
    Sample Types: Serum
  27. Peripheral blood brain-derived neurotrophic factor level and tyrosine kinase B expression on T lymphocytes in systemic lupus erythematosus: Implications for systemic involvement
    Authors: B Tian, C Yang, J Wang, X Hou, S Zhao, Y Li, P Yang
    Cytokine, 2019-06-27;123(0):154764.
    Species: Human
    Sample Types: Serum
  28. Evaluation of Neurosecretome from Mesenchymal Stem Cells Encapsulated in Silk Fibroin Hydrogels
    Authors: Y Martín-Mar, L Fernández-, MH Sanchez-Re, N Marí-Buyé, FJ Rojo, J Pérez-Rigu, M Ramos, GV Guinea, F Panetsos, D González-N
    Sci Rep, 2019-06-19;9(1):8801.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function
    Authors: P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M
    Brain Res. Bull., 2019-04-16;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Increased Calcium Influx through L-type Calcium Channels in Human and Mouse Neural Progenitors Lacking Fragile X Mental Retardation Protein
    Authors: C Danesi, VS Achuta, P Corcoran, UK Peteri, G Turconi, N Matsui, I Albayrak, V Rezov, A Isaksson, ML Castrén
    Stem Cell Reports, 2018-11-29;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  31. Cell encapsulation enhances antidepressant effect of the mesenchymal stem cells and counteracts depressive-like behavior of treatment-resistant depressed rats
    Authors: K Kin, T Yasuhara, M Kameda, Y Tomita, M Umakoshi, K Kuwahara, I Kin, N Kidani, J Morimoto, M Okazaki, T Sasaki, N Tajiri, CV Borlongan, I Date
    Mol. Psychiatry, 2018-08-14;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  32. Association of serum BDNF levels and the BDNF Val66Met polymorphism with the sleep pattern in healthy young adults
    Authors: K Saitoh, R Furihata, Y Kaneko, M Suzuki, S Takahashi, M Uchiyama
    PLoS ONE, 2018-06-26;13(6):e0199765.
    Species: Human
    Sample Types: Serum
  33. Expression of brain-derived neurotrophic factor in kidneys from normal and cyclosporine-treated rats
    Authors: YS Tao, SG Piao, YS Jin, JZ Jin, HL Zheng, HY Zhao, SW Lim, CW Yang, C Li
    BMC Nephrol, 2018-03-14;19(1):63.
    Species: Rat
    Sample Types: Urine
  34. Aquarobic exercises improve the serum blood irisin and brain-derived neurotrophic factor levels in elderly women
    Authors: JH Kim, DY Kim
    Exp. Gerontol., 2018-02-06;104(0):60-65.
    Species: Human
    Sample Types: Serum
  35. BDNF levels are associated with autistic traits in the general population
    Authors: N Brondino, L Fusar-Poli, M Rocchetti, F Bertoglio, N Bloise, L Visai, P Politi
    Psychoneuroendocrinology, 2018-01-09;89(0):131-133.
    Species: Human
    Sample Types: Serum
  36. Initial Observations of Salivary Brain-Derived Neurotrophic Factor Levels in Rett Syndrome.
    Authors: Byiers B, Barney C, Ehrhardt M, Panoskaltsis-Mortari A, Feyma T, Beisang A, Symons F
    Pediatr Neurol, 2017-12-08;80(0):88-89.
    Species: Human
    Sample Types: Saliva
  37. Delayed Maturation of Fast-Spiking Interneurons is Rectified by Activation of the TrkB Receptor in the Mouse Model of Fragile X Syndrome
    Authors: T Nomura, TF Musial, JJ Marshall, Y Zhu, CL Remmers, J Xu, DA Nicholson, A Contractor
    J. Neurosci., 2017-10-16;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  38. Pregnancy promotes pituitary tumors by increasing the rate of the cell cycle
    Authors: C Yin, X Qi
    Oncol Lett, 2017-08-16;14(4):4873-4877.
    Species: Mouse
    Sample Types: Serum
  39. Aberrant cognitive phenotypes and altered hippocampal BDNF expression related to epigenetic modifications in mice lacking the post-synaptic scaffolding protein SHANK1: Implications for autism spectrum disorder
    Authors: AÖ Sungur, MCE Jochner, H Harb, A K?l?ç, H Garn, RKW Schwarting, M Wöhr
    Hippocampus, 2017-05-29;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  40. Elevated glutamate and lactate predict brain death after severe head trauma
    Authors: MA Stefani, R Modkovski, G Hansel, ER Zimmer, A Kopczynski, AP Muller, NR Strogulski, MS Rodolphi, RK Carteri, AP Schmidt, JP Oses, DH Smith, LV Portela
    Ann Clin Transl Neurol, 2017-05-04;4(6):392-402.
    Species: Human
    Sample Types: CSF
  41. Maternal low-protein diet decreases brain-derived neurotrophic factor expression in the brains of the neonatal rat offspring
    Authors: G Marwarha, K Claycombe-, J Schommer, O Ghribi
    J. Nutr. Biochem., 2017-04-06;45(0):54-66.
    Species: Rat
    Sample Types: Tissue Homogenates
  42. Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid
    Authors: SW Saliba, EL Vieira, RP Santos, E Candelario, BL Fiebich, LB Vieira, AL Teixeira, AC de Oliveir
    J Neuroinflammation, 2017-01-31;14(1):25.
    Species: Mouse
    Sample Types: Tissue Homogenates
  43. Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine
    Authors: Xiao-Su Yang
    Biol Open, 2017-01-15;0(0):.
    Species: Rat
    Sample Types: Serum

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Total BDNF Quantikine ELISA Kit
By Anonymous on 11/10/2023
Sample Tested: Cell culture supernatant
Total BDNF Quantikine ELISA Kit
By Anonymous on 11/29/2021
Sample Tested: human
Total BDNF Quantikine ELISA Kit
By Anonymous on 11/23/2021
Sample Tested: Serum
Total BDNF Quantikine ELISA Kit
By Anonymous on 07/25/2019
Sample Tested: rat cortex,rat hippocampus
Total BDNF Quantikine ELISA Kit DBNT00