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Universal Sulfotransferase Activity Kit

Catalog #: EA003
5 Citations Datasheet / COA / SDS
Measurement of sulfotransferase activity
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EA003
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Citations (5)
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Universal Sulfotransferase Activity Kit Summary

View Full Assay Principle

The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.

Features

  • Applicable for all sulfotransferases that use PAPS as the donor substrate
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of sulfotransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • CHO cell-expressed Recombinant Mouse IMPAD1
  • PAP
  • Phosphatase Buffer 3
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of CHST7

Activity of CHST7
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The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-Species

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Citations for Universal Sulfotransferase Activity Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Crystal structure of tick tyrosylprotein sulfotransferase reveals the activation mechanism of the tick anticoagulant protein madanin
    Authors: Yoshimura, M;Teramoto, T;Asano, H;Iwamoto, Y;Kondo, M;Nishimoto, E;Kakuta, Y;
    The Journal of biological chemistry  2024-02-12
  2. Novel silkworm (Bombyx mori) sulfotransferase swSULT ST3 is involved in metabolism of polyphenols from mulberry leaves
    Authors: K Yamamoto, N Yamada, S Endo, K Kurogi, Y Sakakibara, M Suiko
    PLoS ONE, 2022-08-04;17(8):e0270804.  2022-08-04
  3. A gut-derived metabolite alters brain activity and anxiety behaviour in mice
    Authors: BD Needham, M Funabashi, MD Adame, Z Wang, JC Boktor, J Haney, WL Wu, C Rabut, MS Ladinsky, SJ Hwang, Y Guo, Q Zhu, JA Griffiths, R Knight, PJ Bjorkman, MG Shapiro, DH Geschwind, DP Holschneid, MA Fischbach, SK Mazmanian
    Nature, 2022-02-14;602(7898):647-653.  2022-02-14
  4. Correlating chemical sensitivity and basal gene expression reveals mechanism of action.
    Authors: Rees M, Seashore-Ludlow B, Cheah J, Adams D, Price E, Gill S, Javaid S, Coletti M, Jones V, Bodycombe N, Soule C, Alexander B, Li A, Montgomery P, Kotz J, Hon C, Munoz B, Liefeld T, Dancik V, Haber D, Clish C, Bittker J, Palmer M, Wagner B, Clemons P, Shamji A, Schreiber S
    Nat Chem Biol, 2015-12-14;12(2):109-16.  2015-12-14
  5. Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using recombinant sulfotransferases.
    Authors: Wu ZL, Prather B, Ethen CM
    Glycobiology, 2010-12-17;21(5):625-33.  2010-12-17

FAQs

  1. Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

    • The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

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