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Viral Dengue Virus 4 NS1 Antibody

Catalog #: MAB9444 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9444-SP
MAB9444-100
Detection of Dengue Virus 4 NS1 by Western Blot.
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Viral Dengue Virus 4 NS1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects recombinant Dengue Virus 4 NS1 in direct and capture ELISAs and Western blots. In direct ELISAs and Western Blots, no cross-reactivity with recombinant Dengue Virus 1, 2 and 3 NS1 proteins was observed.
Source
Monoclonal Mouse IgG2B Clone # 971314
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 4 NS1 protein
Asp1-Ala352
Accession # NP_740318
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Dengue Virus 4 NS1 antibody by Western Blot. View Larger

Detection of Dengue Virus 4 NS1 by Western Blot. Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Dengue Virus 4 NS1 Monoclonal Antibody (Catalog # MAB9444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 4 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dengue Virus 4 NS1

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Long Name
Dengue Virus 4 Dominica/814669/1981 Polyprotein
Entrez Gene IDs
5075729 (Viral)
Alternate Names
DEN-4; Dengue NS1; Dengue pan; Dengue Virus 4 NS1; Dengue virus Serotype 4; Dengue virus Type 4; DENV4; DENV4-NS1

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