Vitamin D3 Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Vitamin D3

25-hydroxy-vitamin D₃ (25(OH)D₃) is synthesized in the liver and is the primary circulating form of vitamin D.  Its blood concentration, which reflects 25(OH)D₃ produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status.  25(OH)D₃ is metabolized to 1 alpha,25(OH)₂D₃ in the  proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase.   The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure.  It has been shown that the active steroid hormone 1 alpha,25(OH)₂D₃ is essential for life in higher animals.  Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D₃, 1 alpha,25(OH)₂D₃, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Steroid Hormone Receptors and Regulators
• Steroid Hormones and Bioactive Lipids