Home / Protease Substrates / Z-LR-AMC Fluorogenic Peptide Substrate

Z-LR-AMC Fluorogenic Peptide Substrate

Catalog #: ES008
8 Citations 1 Review Datasheet / COA / SDS
Substrate for Cathepsin B, Cathepsin L, and Cathepsin V
Catalog # Availability Size / Price Qty
ES008
R&D Systems Fluorogenic Peptide Substrates
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Citations (8)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Related Research Areas

Z-LR-AMC Fluorogenic Peptide Substrate Summary

Product Specifications

Activity
The peptide substrate contains a highly fluorescent 7-amino-4-methyl coumarin (AMC) group that is efficiently quenched by the amide bond formed between its amino group and the carboxyl group of the Arg residue. It can be used to measure the activities of peptidases that are capable of cleaving this amide bond, causing an increase in fluorescence. It is an excellent substrate for many cathespins. For example, it has been used to assay following recombinant human or mouse cathespins (rhCathespins or rmCathespins) followed by Catalog # in parentheses: rhCathepsin B (953-CY), rmCathepsin B (965-CY), rhCathepsin L (952-CY), and rhCathepsin V (1080-CY).
Source

Z-Leu-Arg-AMC. Z: N-carbobenzyloxy; 7-Amino-4-methylcoumarin.
Predicted Molecular Mass
578 Da

Product Datasheets

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ES008

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES008

Formulation Supplied as a stock solution in dimethyl sulfoxide (DMSO) with a concentration of 20 mg/mL or 32 mM.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Citations for Z-LR-AMC Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Role of engineered metal oxide nanoparticle agglomeration in reactive oxygen species generation and cathepsin B release in NLRP3 inflammasome activation and pulmonary toxicity
    Authors: Tina M Sager
    Inhal Toxicol, 2016-12-05;28(14):686-697.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  2. Redundancy between Cysteine Cathepsins in Murine Experimental Autoimmune Encephalomyelitis.
    Authors: Allan E, Yates R
    PLoS ONE, 2015-06-15;10(6):e0128945.
    Species: Human
    Sample Types: Recombinant Protein
  3. Structural analysis of asparaginyl endopeptidase reveals the activation mechanism and a reversible intermediate maturation stage.
    Authors: Zhao L, Hua T, Crowley C, Ru H, Ni X, Shaw N, Jiao L, Ding W, Qu L, Hung L, Huang W, Liu L, Ye K, Ouyang S, Cheng G, Liu Z
    Cell Res, 2014-01-10;24(3):344-58.
    Species: Mouse
    Sample Types: Protein
  4. The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
    Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
    FASEB J., 2010-05-21;24(10):3744-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Enzyme Assay Substrate
  5. Cystatin M/E is a high affinity inhibitor of cathepsin V and cathepsin L by a reactive site that is distinct from the legumain-binding site. A novel clue for the role of cystatin M/E in epidermal cornification.
    Authors: Cheng T, Hitomi K, van Vlijmen-Willems IM, de Jongh GJ, Yamamoto K, Nishi K, Watts C, Reinheckel T, Schalkwijk J, Zeeuwen PL
    J. Biol. Chem., 2006-03-24;281(23):15893-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  6. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
  7. Distinct roles for cysteine cathepsin genes in multistage tumorigenesis.
    Authors: Gocheva V, Zeng W, Ke D, Klimstra D, Reinheckel T, Peters C, Hanahan D, Joyce JA
    Genes Dev., 2006-02-15;20(5):543-56.
    Species: Human
    Sample Types: Buffer
  8. Characterization of cathepsin L secreted by Sf21 insect cells.
    Authors: Johnson GD, Jiang W
    Arch. Biochem. Biophys., 2005-10-21;444(1):7-14.

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Z-LR-AMC Fluorogenic Peptide Substrate
By Anonymous on 09/23/2021

Reason for Rating: Use the Z-LR-AMC as a substrate for cathepsin L activity assay. It works excellent.
Z-LR-AMC Fluorogenic Peptide Substrate ES008