Using Avi-tag Biotinylated Proteins to Determine Protein Interaction Kinetics and Affinity with Surface Plasmon Resonance

R&D Systems Avi-tag Biotinylated Proteins feature biotinylation at a single lysine residue that resides with the Avi-tag, a unique 15 amino acid peptide. The Avi-tag is enzymatically biotinylated by the E. coli biotin ligase BirA. All R&D Systems Avi-tag Biotinylated Proteins undergo rigorous QC testing to ensure high bioactivity and lot-to-lot consistency.

In this application note, learn how R&D Systems Avi-tag biotinylated proteins can be used as an alternative to chemical or amine coupling in surface plasmon resonance (SPR) experiments.