Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF

Catalog #: 177-IR
4 Citations Datasheet / COA / SDS

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177-IR-100

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.03‑0.1 µg/mL in the presence of 10 ng/mL recombinant human IL‑17.

Source

Mouse myeloma cell line, NS0-derived human IL-17 RA/IL-17 R protein

Human IL-17 RA/IL-17 R (Leu33-Trp 320) Accession # Q96F46	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q96F46

N-terminal Sequence
Analysis

Leu33

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60 kDa (monomer)

SDS-PAGE

100 kDa, reducing conditions

Product Datasheets

177-IR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

177-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL‑17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy‑proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappa B and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway.

References

Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

Related Research Areas

Citations for Recombinant Human IL-17RA/IL-17R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Identification and structure-based drug design of cell-active inhibitors of interleukin 17A at a novel C-terminal site
Authors: ER Goedken, MA Argiriadi, JD Dietrich, AM Petros, N Krishnan, SC Panchal, W Qiu, H Wu, H Zhu, AM Adams, PM Bodelle, L Goguen, PL Richardson, PF Slivka, M Srikumaran, AK Upadhyay, B Wu, RA Judge, A Vasudevan, SM Gopalakris, PB Cox, VS Stoll, C Sun
Scientific Reports, 2022-08-26;12(1):14561.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Diverse Human VH antibody fragments with bio-therapeutic properties from the Crescendo Mouse
Authors: Y Teng, JL Young, B Edwards, P Hayes, L Thompson, C Johnston, C Edwards, Y Sanders, M Writer, D Pinto, Y Zhang, M Roode, P Chovanec, L Matheson, AE Corcoran, A Fernandez, L Montoliu, B Rossi, V Tosato, K Gjuracic, D Nikitin, C Bruschi, B McGuinness, T Sandal, M Romanos
N Biotechnol, 2019-10-07;55(0):65-76.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Binding site elucidation and structure guided design of macrocyclic IL-17A antagonists
Sci Rep, 2016-08-16;6(0):30859.
Species: Human
Sample Types: Protein
Applications: FRET
Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide
Sci Rep, 2016-05-17;6(0):26071.
Species: Human
Sample Types: Whole Cells
Applications: Surface Plasmon Resonance