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Recombinant Human DLL1 His-tag Protein, CF

Catalog #: 1818-DL
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1818-DL-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
FAQs
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DLL1 His-tag Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.25-1.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human DLL1 protein
Human DLL1
(Ser22-Gly540) &
(His152-Gly540)
Accession # AAG09716		 DI 6-His tag
N-terminus C-terminus
Accession #
AAG09716
N-terminal Sequence
Analysis
Ser22 & His152
Predicted Molecular Mass
57 kDa and 43 kDa
SDS-PAGE
66 kDa and 55 kDa, reducing conditions

Product Datasheets

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1818-DL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1818-DL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL1

Delta-like protein 1 (DLL1) is a 90 - 100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6 - 11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References
  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Long Name
Delta-like 1
Entrez Gene IDs
28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names
delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Citations for Recombinant Human DLL1 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells
    Authors: Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I;
    Stem cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells
    Authors: B Bohl, A Jabali, J Ladewig, P Koch
    Science Advances, 2022-02-11;8(6):eabl5792.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells
    Authors: M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer
    Oncogene, 2019-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells
    Authors: R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter
    J. Leukoc. Biol., 2018-10-08;105(1):101-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  6. The CD46-Jagged1 interaction is critical for human TH1 immunity.
    Authors: Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C
    Nat Immunol, 2012-10-21;13(12):1213-21.
    Applications: Binding Assay
  7. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012-07-16;181(3):1099-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. MicroRNA-34a suppresses invasion through downregulation of Notch1 and Jagged1 in cervical carcinoma and choriocarcinoma cells.
    Authors: Pang RT, Leung CO, Ye TM, Liu W, Chiu PC, Lam KK, Lee KF, Yeung WS
    Carcinogenesis, 2010-03-29;31(6):1037-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the molecular weight (MW) of your Recombinant Human DLL1 His-tag Protein?

    • The majority of our Recombinant Human DLL1 His-tag Protein contains full-length DLL1, with a minor contribution of a truncated byproduct (as observed in N-terminal sequencing). The sequence of the full length protein is Ser22-Gly540, with an Asp-Ile linker and C-terminal 6-His-tag, with a predicted MW of 57 kDa. The sequence of the truncated form is His152-Gly540, with an Asp-Ile linker and C-terminal 6-His-tag, with a predicted MW of 43 kDa.

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