Recombinant Mouse Endocan Protein, CF Summary
Product Specifications
Optimal dilutions should be determined by each laboratory for each application.
Trp20-Arg184, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1999-EC
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Endocan/ESM-1
Endocan (endothelial cell proteoglycan; also known as endothelial‑cell specific molecule‑1 [ESM‑1]), is a 50 kDa, monomeric, secreted, cysteine‑rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N‑terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N‑linked glycosylation sites. Based on human Endocan, there are at least two potential O‑linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4‑O sulfated N‑acetyl galactosamine with alpha ‑iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF‑ alpha and VEGF, (1, 7) and is known to bind to LFA‑1 ( alpha L beta 2) on the surface of PBMCs, blocking LFA‑1 interaction with ICAM‑1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).
- Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
- Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
- Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
- Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
- Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
- Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
- Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.
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