Home / Angiopoietin-1 / Recombinant Human Angiopoietin-1 Protein

Recombinant Human Angiopoietin-1 Protein

Catalog #: 923-AN
71 Citations 6 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
923-AN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
923-AN-025
Best Seller
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (71)
FAQs
Reviews (6)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Angiopoietin-1 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is 10-40 ng/mL in the presence of 5 µg/mL of a
cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived human Angiopoietin-1 protein
Ser20-Phe498, with a C-terminal 6-His tag
Accession #
Q5HYA0
N-terminal Sequence
Analysis
Ser20
Structure / Form
Oligomer
Predicted Molecular Mass
56 kDa
SDS-PAGE
70 kDa, reducing conditions

Product Datasheets

You must select a language.

x

923-AN (with carrier)

Product Datasheet
COA
SDS

You must select a language.

x

923-AN/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

923-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

923-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Angiopoietin-1

Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system (1, 2). It contains a coiled-coil region and a C-terminal fibrinogen-like domain separated by a short flexible region (3, 4). Mature human Angiopoietin-1 shares 97% amino acid sequence identity with mouse and rat Angiopoietin-1. It is expressed by vascular smooth muscle cells and pericytes as an approximately 70 kDa molecule that associates into
disulfide-linked homotrimers, tetramers, and pentamers (3, 5). Angiopoietin-1 binds and activates the receptor tyrosine kinase Tie-2, and its association into tetramers is important for full Tie-2 activation (3, 4). Angiopoietin-1 ligation of Tie-2 on vascular endothelial cells (EC) induces the development and branching of blood vessels (6, 7). In sub-confluent EC (i.e. during angiogenesis), Angiopoietin-1 promotes EC motility and Tie-2 localization at the trailing edge of the cell (8). In confluent EC (i.e. in homeostasis), multimeric Angiopoietin-1 enhances vascular integrity by promoting the in trans homotypic association of Tie-2 between EC or with the substratum (8, 9). In addition, Angiopoietin-1 suppresses several VEGF-induced effects on the vasculature including endothelial permeability, stretch-induced release of Angiopoietin-2, and up-regulation of the leukocyte adhesion molecules VCAM-1, ICAM-1, and E-Selectin (10-12). Angiopoietin-1 also interacts with a variety of integrins and the extracellular matrix independently of Tie-2 (13, 14). These interactions support the adhesion, migration and stress resistance of EC, fibroblasts, and myocytes (13, 14). Angiopoietin-1 can protect against pulmonary arterial hypertension (5), reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium (15), and enhance tumor progression and metastasis (16).

References
  1. Koh, G.Y. (2012) Trends Mol. Med. 19:31.
  2. Suri, C. et al. (1996) Cell 87:1171.
  3. Davis, S. et al. (1996) Cell 87:1161.
  4. Kim, K.-T. et al. (2005) J. Biol. Chem. 280:20126.
  5. Kugathasan, L. et al. (2009) J. Exp. Med. 206:2221.
  6. Suri, C. et al. (1998) Science 282:468.
  7. Jeansson, M. et al. (2011) J. Clin. Invest. 121:2278.
  8. Saharinen, P. et al. (2008) Nat. Cell. Biol. 10:527.
  9. Fukuhara, S. et al. (2008) Nat. Cell Biol. 10:513.
  10. Jho, D. et al. (2005) Circ. Res. 96:1282.
  11. Korff, T. et al. (2012) Cardiovasc. Res. 94:510.
  12. Kim, I. et al. (2001) Circ. Res. 89:477.
  13. Carlson, T.R. et al. (2001) J. Biol. Chem. 276:26516.
  14. Dallabrida, S.M. et al. (2005) Cardiovasc. Res. 96:e8.
  15. Samuel, S.M. et al. (2010) Diabetes 59:51.
  16. Holopainen, T. et al. (2009) Cancer Res. 69:4656.
Entrez Gene IDs
284 (Human); 11600 (Mouse)
Alternate Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003

Citations for Recombinant Human Angiopoietin-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

71 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Glycation of fibronectin impairs angiopoietin-1/Tie-2 signaling through uncoupling Tie-2-?5?1 integrin crosstalk
    Authors: Chen, T;Zhou, H;Yuan, S;Deng, X;Li, Y;Chen, N;You, J;Li, R;Li, T;Zheng, Y;Luo, M;Lv, H;Wu, J;Wang, L;
    Cellular signalling
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. An optogenetic-phosphoproteomic study reveals dynamic Akt1 signaling profiles in endothelial cells
    Authors: Zhou, W;Li, W;Wang, S;Salovska, B;Hu, Z;Tao, B;Di, Y;Punyamurtula, U;Turk, BE;Sessa, WC;Liu, Y;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
    Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
    Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Angiotensin-converting enzyme inhibitor promotes angiogenesis through Sp1/Sp3-mediated inhibition of notch signaling in male mice
    Authors: H Lu, P Yuan, X Ma, X Jiang, S Liu, C Ma, S Philipsen, Q Zhang, J Yang, F Xu, C Zhang, Y Zhang, W Zhang
    Nature Communications, 2023-02-09;14(1):731.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. TIE2-high cervical cancer cells promote tumor angiogenesis by upregulating TIE2 and VEGFR2 in endothelial cells
    Authors: S Tan, Y Chen, S Du, W Li, P Liu, J Zhao, P Yang, J Cai, R Gao, Z Wang
    Translational Oncology, 2022-09-15;26(0):101539.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis
    Authors: H Zhou, T Chen, Y Li, J You, X Deng, N Chen, T Li, Y Zheng, R Li, M Luo, J Wu, L Wang
    International Journal of Molecular Sciences, 2022-06-27;23(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
    Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
    PloS Pathogens, 2022-01-31;18(1):e1010280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Quantitative Analysis of Factors Regulating Angiogenesis for Stem Cell Therapy
    Authors: T Shimazaki, N Noro, K Hagikura, T Matsumoto, C Yoshida-No
    Biology, 2021-11-20;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner
    Authors: R Luck, A Karakatsan, B Shah, G Schermann, H Adler, J Kupke, N Tisch, HW Jeong, MK Back, F Hetsch, A D'Errico, M De Palma, E Wiedtke, D Grimm, A Acker-Palm, J von Engelh, RH Adams, HG Augustin, C Ruiz de Al
    Cell Reports, 2021-08-17;36(7):109522.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  10. An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1
    Authors: Y Koya, H Nara, S Yagi, C Ueno, M Kamohara
    Scientific Reports, 2021-07-07;11(1):14021.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Mucopolysaccharide polysulfate promotes microvascular stabilization and barrier integrity of dermal microvascular endothelial cells via activation of the angiopoietin-1/Tie2 pathway
    Authors: S Fujiwara-S, Y Ueda, M Fujikawa, M Osaki, N Yamanaka, T Matsumoto
    Journal of dermatological science, 2021-06-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
    Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
    Nature Neuroscience, 2021-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Influence of Stress and Depression on the Immune System in Patients Evaluated in an Anti-aging Unit
    Authors: B Cañas-Gonz, A Fernández-, JM Ramírez, V Martínez-F
    Front Psychol, 2020-08-04;11(0):1844.
    Species: Human
    Sample Types: Whole Cell, Whole Cells
    Applications: Bioassay
  14. Roles of miR-640 and Zinc Finger Protein 91 (ZFP91) in Angiopoietin-1-Induced In Vitro Angiogenesis
    Authors: S Harel, V Sanchez-Go, R Echavarria, D Mayaki, SN Hussain
    Cells, 2020-07-02;9(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  15. Id1 and Id3 Maintain Steady-State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration
    Authors: S Gadomski, SK Singh, S Singh, T Sarkar, KD Klarmann, M Berenschot, S Seaman, B Jakubison, KO Gudmundsso, S Lockett, JR Keller
    Cell Rep, 2020-04-28;31(4):107572.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody
    Authors: M Singhal, N Gengenbach, S La Porta, S Gehrs, J Shi, M Kamiyama, DM Bodenmille, A Fischl, B Schieb, E Besemfelde, S Chintharla, HG Augustin
    EMBO Mol Med, 2020-04-17;0(0):e11164.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Rapid Development of Glaucoma Via ITV Nonselective ANGPT 1/2 Antibody: A Potential Role for ANGPT/TIE2 Signaling in Primate Aqueous Humor Outflow
    Authors: EA Thackaberr, Y Zhou, CL Zuch de Za, G Fuh, CV Lee, S Sanowar, JB Ridgway, AM Kusi, C Farman, H Booler, D Sheinson, CA Rasmussen, PE Miller, E Wakshull, M Yan, V Bantseev
    Invest. Ophthalmol. Vis. Sci., 2019-10-01;60(13):4097-4108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. IGF-1R deficiency in human keratinocytes disrupts epidermal homeostasis and stem cell maintenance
    Authors: T Muraguchi, D Nanba, EK Nishimura, T Tashiro
    J. Dermatol. Sci., 2019-05-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Growth Factor-like Gene Regulation Is Separable from Survival and Maturation in Antibody-Secreting Cells
    Authors: S Stephenson, MA Care, I Fan, A Zougman, DR Westhead, GM Doody, RM Tooze
    J. Immunol., 2019-01-14;202(4):1287-1300.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
    Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
    Elife, 2018-11-16;7(0):.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, ELISA (Capture), Surface Plasmon Resonance
  21. Tie2 protects the vasculature against thrombus formation in systemic inflammation
    Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
    J. Clin. Invest., 2018-03-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Context-dependent functions of angiopoietin 2 are determined by the endothelial phosphatase VEPTP
    Authors: T Souma, BR Thomson, S Heinen, IA Carota, S Yamaguchi, T Onay, P Liu, AK Ghosh, C Li, V Eremina, YK Hong, AN Economides, D Vestweber, KG Peters, J Jin, SE Quaggin
    Proc. Natl. Acad. Sci. U.S.A., 2018-01-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Endothelial Tie1-mediated angiogenesis and vascular abnormalization promote tumor progression and metastasis
    Authors: S La Porta, L Roth, M Singhal, C Mogler, C Spegg, B Schieb, X Qu, RH Adams, HS Baldwin, S Savant, HG Augustin
    J. Clin. Invest., 2018-01-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor
    Authors: C Daly, X Qian, C Castanaro, E Pasnikowsk, X Jiang, BR Thomson, SE Quaggin, N Papadopoul, Y Wei, JS Rudge, G Thurston, GD Yancopoulo, S Davis
    Sci Rep, 2018-01-11;8(1):505.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Dachengqi Decoction Attenuates Intestinal Vascular Endothelial Injury in Severe Acute Pancreatitis in Vitro and in Vivo
    Authors: LY Pan, YF Chen, HC Li, LM Bi, WJ Sun, GF Sun, XF Zhang, K Xu, DX Feng
    Cell. Physiol. Biochem., 2017-12-18;44(6):2395-2406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Bicarbonate buffered peritoneal dialysis fluid upregulates angiopoietin-1 and promotes vessel maturation
    Authors: G Eich, M Bartosova, C Tischer, TT Wlodkowski, B Schaefer, S Pichl, N Kraewer, B Ranchin, K Vondrak, MC Liebau, T Hackert, CP Schmitt
    PLoS ONE, 2017-12-18;12(12):e0189903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Vasculotide reduces pulmonary hyperpermeability in experimental pneumococcal pneumonia
    Authors: B Gutbier, X Jiang, K Dietert, C Ehrler, J Lienau, P Van Slyke, H Kim, VC Hoang, JT Maynes, DJ Dumont, AD Gruber, N Weissmann, TJ Mitchell, N Suttorp, M Witzenrath
    Crit Care, 2017-11-13;21(1):274.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. CD146 coordinates brain endothelial cell-pericyte communication for blood-brain barrier development
    Authors: J Chen, Y Luo, H Hui, T Cai, H Huang, F Yang, J Feng, J Zhang, X Yan
    Proc. Natl. Acad. Sci. U.S.A., 2017-08-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Angiopoietin-1 enhances neutrophil chemotaxis in vitro and migration in vivo through interaction with CD18 and release of CCL4
    Authors: A Burnett, I Gomez, DD De Leon, M Ariaans, P Progias, RA Kammerer, G Velasco, M Marron, P Hellewell, V Ridger
    Sci Rep, 2017-05-24;7(1):2332.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy
    Authors: T Shlamkovic, L Aharon, WA Barton, N Papo
    Oncotarget, 2017-05-16;8(20):33571-33585.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  31. Dimerization of Tie2 mediated by its membrane-proximal FNIII domains
    Authors: JO Moore, MA Lemmon, KM Ferguson
    Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration
    Authors: K Wang, W Liu, Y Song, X Wu, Y Zhang, S Li, Y Gao, J Tu, Y Liu, C Yang
    Lab. Invest., 2017-04-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
    Authors: YT Ho, G Adriani, S Beyer, PT Nhan, RD Kamm, JC Kah
    Sci Rep, 2017-03-31;7(1):707.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
    Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
    Circ. Res, 2017-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. G protein-coupled receptor 34 in ovarian granulosa cells of cattle: changes during follicular development and potential functional implications
    Authors: L J Spicer
    Domest. Anim. Endocrinol, 2016-12-11;59(0):90-99.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats
    Sci Rep, 2016-09-19;6(0):33428.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  37. New Insights into the Pro-Inflammatory Activities of Ang1 on Neutrophils: Induction of MIP-1? Synthesis and Release
    PLoS ONE, 2016-09-15;11(9):e0163140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Tie1 controls angiopoietin function in vascular remodeling and inflammation
    J Clin Invest, 2016-08-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells
    Authors: Wilson Chan
    PLoS ONE, 2016-06-15;11(6):e0156994.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. TIE2-mediated tyrosine phosphorylation of H4 regulates DNA damage response by recruiting ABL1
    Sci Adv, 2016-04-01;2(4):e1501290.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Acute Systemic Infection with Dengue Virus Leads to Vascular Leakage and Death through Tumor Necrosis Factor-alpha and Tie2/Angiopoietin Signaling in Mice Lacking Type I and II Interferon Receptors.
    Authors: Phanthanawiboon S, Limkittikul K, Sakai Y, Takakura N, Saijo M, Kurosu T
    PLoS ONE, 2016-02-04;11(2):e0148564.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  42. Angiopoietin-1 Regulates Brain Endothelial Permeability through PTPN-2 Mediated Tyrosine Dephosphorylation of Occludin.
    Authors: Siddiqui M, Mayanil C, Kim K, Tomita T
    PLoS ONE, 2015-06-19;10(6):e0130857.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
    Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
    Nat Commun, 2015-01-30;6(0):5962.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways.
    Authors: Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, Brantley-Sieders D, Chen J
    Mol Cell Biol, 2015-01-12;35(7):1299-313.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Vasculotide, an Angiopoietin-1 mimetic, reduces acute skin ionizing radiation damage in a preclinical mouse model.
    Authors: Korpela, Elina, Yohan, Darren, Chin, Lee Cl, Kim, Anthony, Huang, Xiaoyong, Sade, Shachar, Van Slyke, Paul, Dumont, Daniel J, Liu, Stanley
    BMC Cancer, 2014-08-26;14(0):614.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment.
    Authors: Brunckhorst M, Xu Y, Lu R, Yu Q
    Am J Pathol, 2014-08-01;184(8):2285-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. The RNA binding protein hnRNP-K mediates post-transcriptional regulation of uncoupling protein-2 by angiopoietin-1.
    Authors: Tahir T, Singh H, Brindle N
    Cell Signal, 2014-03-15;26(7):1379-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Angiopoietin1 inhibits mast cell activation and protects against anaphylaxis.
    Authors: Yao J, Cui M, Li M, Liu Y, He Q, Xiao J, Bai Y
    PLoS ONE, 2014-02-19;9(2):e89148.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils.
    Authors: Haddad L, Sirois M
    PLoS ONE, 2014-02-12;9(2):e88980.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
    Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
    PLoS ONE, 2014-01-15;9(1):e85655.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1.
    Authors: Pellegrinelli V, Rouault C, Veyrie N, Clement K, Lacasa D
    Diabetes, 2013-10-15;63(2):535-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Directed evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display.
    Authors: Brindle, Nicholas, Sale, Julian E, Arakawa, Hiroshi, Buerstedde, Jean-Mar, Nuamchit, Teonchit, Sharma, Shikha, Steele, Kathryn
    J Biol Chem, 2013-10-08;288(46):33205-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Comparative analysis of human mesenchymal stem cells from bone marrow, adipose tissue, and umbilical cord blood as sources of cell therapy.
    Authors: Jin H, Bae Y, Kim M, Kwon S, Jeon H, Choi S, Kim S, Yang Y, Oh W, Chang J
    Int J Mol Sci, 2013-09-03;14(9):17986-8001.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Structural basis for angiopoietin-1-mediated signaling initiation.
    Authors: Yu, Xuehong, Seegar, Tom C M, Dalton, Annamari, Tzvetkova-Robev, Dorothea, Goldgur, Yehuda, Rajashankar, Kanagala, Nikolov, Dimitar, Barton, William
    Proc Natl Acad Sci U S A, 2013-04-16;110(18):7205-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Ligand-independent Tie2 dimers mediate kinase activity stimulated by high dose angiopoietin-1.
    Authors: Yamakawa, Daishi, Kidoya, Hiroyasu, Sakimoto, Susumu, Jia, Weizhen, Naito, Hisamich, Takakura, Nobuyuki
    J Biol Chem, 2013-03-15;288(18):12469-77.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Integration and regression of implanted engineered human vascular networks during deep wound healing.
    Authors: Hanjaya-Putra D, Shen Y, Wilson A, Fox-Talbot K, Khetan S, Burdick J, Steenbergen C, Gerecht S
    Stem Cells Transl Med, 2013-03-13;2(4):297-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Acute administration of recombinant Angiopoietin-1 ameliorates multiple-organ dysfunction syndrome and improves survival in murine sepsis.
    Authors: David S, Park JK, Meurs M, Zijlstra JG, Koenecke C, Schrimpf C, Shushakova N, Gueler F, Haller H, Kumpers P
    Cytokine, 2011-04-30;55(2):251-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  58. Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
    Authors: Brunckhorst MK, Wang H, Lu R, Yu Q
    Cancer Res., 2010-09-07;70(18):7283-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
    Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
    J. Biol. Chem., 2009-08-12;284(42):29109-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
    Authors: Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
    FASEB J., 2008-05-23;22(8):3010-23.
    Applications: Western Blot
  61. Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
    Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
    Nat. Cell Biol., 2008-04-20;10(5):527-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. Obligatory participation of macrophages in an angiopoietin 2-mediated cell death switch.
    Authors: Rao S, Lobov IB, Vallance JE, Tsujikawa K, Shiojima I, Akunuru S, Walsh K, Benjamin LE, Lang RA
    Development, 2007-12-01;134(24):4449-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  63. Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
    Authors: Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC
    Clin. Immunol., 2007-06-08;124(2):158-64.
    Applications: ELISA (Standard)
  64. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007-04-19;25(8):1862-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. A neurovascular niche for neurogenesis after stroke.
    Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
    J. Neurosci., 2006-12-13;26(50):13007-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  66. Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo.
    Authors: Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C
    J. Clin. Invest., 2006-05-04;116(6):1596-605.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  67. Regulation of Ang2 release by PTEN/PI3-kinase/Akt in lung microvascular endothelial cells.
    Authors: Tsigkos S, Zhou Z, Kotanidou A, Fulton D, Zakynthinos S, Roussos C, Papapetropoulos A
    J. Cell. Physiol., 2006-05-01;207(2):506-11.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  68. Angiopoietin-2 levels are elevated in exudative pleural effusions.
    Authors: Kalomenidis I, Kollintza A, Sigala I, Papapetropoulos A, Papiris S, Light RW, Roussos C
    Chest, 2006-05-01;129(5):1259-66.
    Applications: ELISA (Standard)
  69. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004-03-01;15(3):566-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1.
    Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY
    Eur. J. Clin. Invest., 2003-10-01;33(10):883-90.
    Applications: ELISA (Standard)
  71. Expansion of human SCID-repopulating cells under hypoxic conditions.
    Authors: Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
    J. Clin. Invest., 2003-07-01;112(1):126-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Angiopoietin-1 Protein

Average Rating: 4.5 (Based on 6 Reviews)

5 Star
66.67%
4 Star
16.67%
3 Star
16.67%
2 Star
0%
1 Star
0%

Have you used Recombinant Human Angiopoietin-1 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Human Angiopoietin-1 Protein
By Joseph Ogbede on 04/10/2024
Application: In vitro bioactivity in cell culture
Reason for Rating: Worked well for my experiment. I used it to activate TIE2 and AKT in HUVEC. Lane 1 is control treated cells; Lane 2 is rh ANGPT1 (923-AN) treated cells. As can be seen, there is activation (phosphorylation) of AKT in the 923-AN treated cells.
Recombinant Human Angiopoietin-1 Protein
By Sarah Garrett on 11/22/2019
Application: In vitro bioactivity in cell culture

HMEC-1 cells were grown to confluence and then serum starved overnight. The next days cells were washed and either left untreated (lane 1) or stimulated with 600 ng/mL Ang-1 for 15min (lane 2). Cells were scraped and analyzed by western blot for AKT and pAKT and showed clear And-1 dependent AKT activation.

In vitro bioactivity in cell culture Recombinant Human Angiopoietin-1 Protein 923-AN
Recombinant Human Angiopoietin-1 Protein
By Anonymous on 09/03/2019
Application: SDS-PAGE Control
SDS-PAGE Control Recombinant Human Angiopoietin-1 Protein 923-AN
Recombinant Human Angiopoietin-1 Protein
By Mohammad Daud Khan on 10/16/2018
Application: endothelial cells clustering Tie2 when being treated with RH Ang1
Other Recombinant Human Angiopoietin-1 Protein 923-AN
Recombinant Human Angiopoietin-1 Protein
By Anonymous on 12/06/2017
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human Angiopoietin-1 Protein 923-AN
Recombinant Human Angiopoietin-1 Protein
By Anonymous on 12/03/2017
Application: SDS-PAGE Control
Reason for Rating: It performed as expected.
SDS-PAGE Control Recombinant Human Angiopoietin-1 Protein 923-AN