Human Neprilysin/CD10 Antibody

Catalog #: AF1182
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1182
AF1182-SP

2 Images

Product Details

Citations (5)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neprilysin/CD10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neprilysin/CD10 in ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10
Tyr52-Trp750
Accession # P08473

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

CD19⁺ human peripheral blood lymphocytes

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Neprilysin/CD10 (Catalog # 1182-ZNC), see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human Neprilysin/CD10 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Human Neprilysin/CD10 Biotinylated Antibody (Catalog # BAF1182)

Standard: Recombinant Human Neprilysin (CHO-expressed) Protein, CF (Catalog # 1182-ZNC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Neprilysin/CD10 in Human Kidney. Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

View Larger

Detection of Human and Mouse Neprilysin/CD10 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Neprilysin/CD10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1182) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, Recombinant Mouse Neprilysin (1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin/CD10

Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide (1, 2). NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states (3, 4). These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (A beta ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting A beta accumulation (5).

References

Malfroy, B. et al. (1978) Nature 276:523.
Kenny, A.J. and Stephenson, S.L. (1988) FEBS Lett. 232:1.
LeTarte, M. et al. (1988) J. Exp. Med. 168:1247.
Shipp, M.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4819.
Itwata, N. et al. (2001) Science 292:1550.

Entrez Gene IDs

4311 (Human); 17380 (Mouse); 24590 (Rat); 101925772 (Cynomolgus Monkey)

Alternate Names

Atriopeptidase; CALLA; CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); CD10 antigen; CD10; CD10); CD10membrane metallo-endopeptidase variant 1; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24; EC 3.4.24.11; Enkephalinase; EPN; Leu-19; membrane metallo-endopeptidase variant 2; membrane metallo-endopeptidase; MGC126681; MGC126707; MME; NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA; Neprilysin; Neutral Endopeptidase 24.11; Neutral endopeptidase; NKH1; SFE; Skin fibroblast elastase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Neprilysin/CD10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

Seminal-Plasma-Mediated Effects on Sperm Performance in Humans
Authors: T Turunen, M Magris, M Malinen, J Kekäläinen
Cells, 2022-07-08;11(14):.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot
Neuroprotection induced by Navbeta2?knockdown in APP/PS1 transgenic neurons is associated with NEP regulation
Authors: T Hu, SS Li, MN Lu, L Zhang, B Chen, R Mao, R Mei, YX Tan, S Li, YB Xiyang
Mol Med Rep, 2019-06-20;20(2):2002-2011.
Species: Mouse
Sample Types: Cell Lysates
Applications: Activity Assay
Vitamin D and Its Analogues Decrease Amyloid-? (A?) Formation and Increase A?-Degradation
Authors: MOW Grimm, A Thiel, AA Lauer, J Winkler, J Lehmann, L Regner, C Nelke, D Janitschke, C Benoist, O Streidenbe, H Stötzel, K Endres, C Herr, C Beisswenge, HS Grimm, R Bals, F Lammert, T Hartmann
Int J Mol Sci, 2017-12-19;18(12):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Activity Assay
HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes.
Authors: Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T
J. Immunol., 2011-05-06;186(12):6925-32.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ELISA Development, ICC
Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin.
Authors: Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M, Schmitt AL, Verleysdonk S, Kabisch D, Koeppen K, Siegel G, Proksch B, Kluba T, Eckert A, Kohle C, Schoneberg T, Northoff H, Schwab M, Gleiter CH
Cell Death Differ., 2009-07-17;16(12):1599-614.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot