Home / Neuroplastin 65 / Human/Mouse Neuroplastin 65 Antibody

Human/Mouse Neuroplastin 65 Antibody

Catalog #: AF5360
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5360
AF5360-SP
Neuroplastin 65 in Human Brain.
2 Images
Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Neuroplastin 65 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Neuroplastin 65 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neuroplastin 55 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 65
Gln29-His336
Accession # NP_036560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Neuroplastin 65 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Neuroplastin 65 in Human Brain. Neuroplastin 65 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 3 µg/mL Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human/Mouse Neuroplastin 65 antibody by Western Blot. View Larger

Detection of Human/Mouse Neuroplastin 65 by Western Blot. Western blot shows lysates of human brain and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Neuroplastin 65 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroplastin 65 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroplastin 65

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52-57 kDa member of the Ig-superfamily. It is widely expressed and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32-119 and 122-213) and a 38 aa cytoplasmic region (aa 245-282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N terminal 116 aa Ig-like domain-containing insert after Asn30 and interacts homotypically. Notably, human and rodent Np65 expression patterns are different in brain, with rodent Np65 not predominating in cerebellum. Over aa 29-336, human Np65 is 95% aa identical to mouse Np65.

Entrez Gene IDs
27020 (Human); 56064 (Rat)
Alternate Names
GP55;GP65;np55;np65;NPTN;SDFR1;SDR1; Neuroplastin 65; Np65

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Citations for Human/Mouse Neuroplastin 65 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function
    J Neurosci, 2016-08-31;36(35):9201-16.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Neuroplastin-65 and a mimetic peptide derived from its homophilic binding site modulate neuritogenesis and neuronal plasticity.
    Authors: Owczarek S, Soroka V, Kiryushko D, Larsen MH, Yuan Q, Sandi C, Berezin V, Bock E
    J. Neurochem., 2011-05-13;117(6):984-94.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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