Human/Primate GDF-15 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF940
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human/Primate GDF-15 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human GDF-15 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF-5, rmGDF-6, rmGDF-7, and rmGDF-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GDF-15 (R&D Systems, Catalog # 957-GD)
Ala197-Ile308
Accession # Q99988
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GDF‑15 (Catalog # 957-GD)

Human/Primate GDF-15 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate GDF‑15 Antibody (Catalog # MAB957)

Standard: Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog # 957-GD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

References
  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
Long Name
Growth Differentiation Factor 15
Entrez Gene IDs
9518 (Human); 23886 (Mouse); 29455 (Rat)
Alternate Names
GDF15; GDF-15; growth differentiation factor 15; growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC-1; MIC-1NSAID-activated gene 1 protein; MIC1Prostate differentiation factor; NAG-1; NAG-1NSAID-regulated gene 1 protein; NSAID (nonsteroidal inflammatory drug)-activated protein 1; PDF; PDFGDF-15; PLAB; PLABNRG-1; Placental bone morphogenetic protein; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

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Citations for Human/Primate GDF-15 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Circulating levels of GDF15 in patients with myalgic encephalomyelitis/chronic fatigue syndrome
    Authors: A Melvin, E Lacerda, HM Dockrell, S O'Rahilly, L Nacul
    J Transl Med, 2019-12-04;17(1):409.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  2. GDF15/MIC1 and MMP9 Cerebrospinal Fluid Levels in Parkinson's Disease and Lewy Body Dementia.
    Authors: Walter Maetzler, Willy Deleersni, Valérie Hanssens, Alice Bernard, Kathrin Brockmann, Justus Marquetan, Isabel Wurster, Tim W Rattay, Lorenzo Roncoroni, Eva Schaeffer, Stefanie Lerche, Anja Apel, Christian Deuschle, Daniela Berg
    PLoS ONE, 2016-03-03;0(0):1932-6203.
    Species: Human
    Sample Types: Beads
    Applications: Luminex Development
  3. Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
    Authors: Li, Ji, Veltri, Robert W, Yuan, Zhen, Christudass, Christhu, Mandecki, Wlodek
    PLoS ONE, 2015-04-08;10(4):e0122249.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  4. AF1q: A Novel Mediator of Basal and 4-HPR-Induced Apoptosis in Ovarian Cancer Cells.
    Authors: Tiberio P, Cavadini E, Callari M
    PLoS ONE, 2012-06-26;7(6):e39968.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Growth differentiation factor-15 predicts mortality and morbidity after cardiac resynchronization therapy.
    Authors: Foley PW, Stegemann B, Ng K, Ramachandran S, Proudler A, Frenneaux MP, Ng LL, Leyva F
    Eur. Heart J., 2009-08-07;30(22):2749-57.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  6. Growth differentiation factor-15 as a prognostic marker in patients with acute myocardial infarction.
    Authors: Khan SQ, Ng K, Dhillon O, Kelly D, Quinn P, Squire IB, Davies JE, Ng LL
    Eur. Heart J., 2009-01-23;30(9):1057-65.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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Reviews for Human/Primate GDF-15 Biotinylated Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human/Primate GDF-15 Biotinylated Antibody
By Anonymous on 11/08/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the detection in an ELISA along with MAB957 as the capture antibody. Human serum and plasma samples were measurable.


Human/Primate GDF-15 Biotinylated Antibody
By Anonymous on 06/11/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

This antibody was used as the pair to MAB957 in an GDF-15 immunoassay.