Human L-Selectin/CD62L Antibody

Catalog #: BBA24
7 Citations Datasheet / COA / SDS

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BBA24

Product Details

Citations (7)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human L-Selectin/CD62L Antibody Summary

Species Reactivity

Human

Specificity

Binds to CHO cells expressing the human L‑Selectin/CD62L protein. It does not recognize CHO cells expressing either the human E-Selectin or human P-Selectin.

Source

Monoclonal Mouse IgG₁ Clone # 4G8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human L‑Selectin/CD62L
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood lymphocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human L-Selectin/CD62L Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human L-Selectin/CD62L Biotinylated Antibody (Catalog # BAF728)

Standard: Recombinant Human L-Selectin/CD62L Protein, CF (Catalog # ADP2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L-Selectin/CD62L

L-Selectin (LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. It plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites.

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Alternate Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human L-Selectin/CD62L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Augmentation of Human Monocyte Responses to Lipopolysaccharide by the Protein S and Mer/Tyro3 Receptor Tyrosine Kinase Axis
Authors: ND Barth, JA Marwick, MJ Heeb, AJ Gale, AG Rossi, I Dransfield
J. Immunol., 2018-09-24;0(0):.
NFKB1 regulates human NK cell maturation and effector functions
Authors: Vassilios Lougaris
Clin. Immunol, 2016-12-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation.
Authors: Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont
Scientific Reports, 2016-02-26;0(0):2045-2322.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Facile supermolecular aptamer inhibitors of L-selectin.
Authors: Chang, Elizabet, Eckert, Mark A, Ali, M Monsur, Riazifar, Hamidrez, Pone, Egest J, Liu, Linan, Zhao, Weian
PLoS ONE, 2015-03-31;10(3):e0123034.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development
Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
J. Immunol., 2011-12-16;188(3):1002-10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology, 2004-06-01;112(2):274-89.
Species: Human
Sample Types:
Applications: Flow Cytometry
The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
Immunity, 2003-01-01;18(1):87-96.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry