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Mouse ST2/IL-33R Antibody

Catalog #: MAB10041
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10041-SP
MAB10041-100
MAB10041-500
Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry.
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Citations (11)
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Mouse ST2/IL-33R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ST2/IL-33 R in ELISAs.
Source
Monoclonal Rat IgG2B Clone # 245707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Arg332
Accession # P14719
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Mouse ST2/IL-33 R Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse ST2/IL-33R Biotinylated Antibody (Catalog # BAF1004)

Standard: Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF (Catalog # 1004-MR)

Neutralization
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. Schmitz, J. et al. (2005) Immunity 23:479. The Neutralization Dose (ND50) is typically 0.5-3 ug/mL in the presence of 1 ng/mL Recombinant Human IL‑33.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ST2/IL-33 R antibody in P815 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry. P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Neutralization Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. View Larger

Cell Proliferation Induced by IL‑33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL-33 induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND50 is typically 0.5-3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).

References
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Schmitz, J. et al. (2005) Immunity 23:479.
  5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
  9. Palmer, G. et al. (2008) Cytokine 42:358.
  10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name
Interleukin 33 Receptor
Entrez Gene IDs
9173 (Human); 17082 (Mouse)
Alternate Names
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

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Citations for Mouse ST2/IL-33R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Interleukin-33 Induces Protective Effects in Adipose Tissue Inflammation During Obesity in Mice
    Authors: Ashley M. Miller, Darren L. Asquith, Axel J. Hueber, Lesley A. Anderson, William M. Holmes, Andrew N. McKenzie et al.
    Circulation Research
  2. sST2: A Bridge Between Sirt1/p53/p21 Signal-Induced Senescence and TGF-beta 1/Smad2/3 Regulation of Cardiac Fibrosis in Mouse Viral Myocarditis
    Authors: Jiajia Tan, Jing Wei, Hongxiang Lu
    Inflammation
  3. Hyperactivity of Innate Immunity Triggers Pain via TLR2-IL-33-Mediated Neuroimmune Crosstalk
    Authors: J Huang, MA Gandini, L Chen, S M'Dahoma, PL Stemkowski, H Chung, DA Muruve, GW Zamponi
    Cell Rep, 2020-10-06;33(1):108233.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Mast cells are essential intermediaries in regulating IL-33/ST2 signaling for an immune network favorable to mucosal healing in experimentally inflamed colons
    Authors: Z He, J Song, J Hua, M Yang, Y Ma, T Yu, J Feng, B Liu, X Wang, Y Li, J Li
    Cell Death Dis, 2018-12-05;9(12):1173.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Mechanism for Initiation of Food Allergy: Dependence on skin barrier mutations and environmental allergen co-stimulation
    Authors: M Walker, J Green, R Ferrie, A Queener, MH Kaplan, JM Cook-Mills
    J. Allergy Clin. Immunol., 2018-02-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
    Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
    J Innate Immun, 2018-01-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. CD301b(+) dermal dendritic cells drive T helper 2 cell-mediated immunity.
    Authors: Kumamoto Y, Linehan M, Weinstein J, Laidlaw B, Craft J, Iwasaki A
    Immunity, 2013-09-26;39(4):733-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
    Authors: Monticelli LA, Sonnenberg GF, Abt MC
    Nat. Immunol., 2011-11-01;12(11):1045-54.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Paracrine IL-33 Stimulation Enhances Lipopolysaccharide-Mediated Macrophage Activation.
    Authors: Ohno T, Oboki K, Morita H
    PLoS ONE, 2011-04-11;6(4):e18404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  11. Adiponectin restrains ILC2 activation by AMPK-mediated feedback inhibition of IL-33 signaling
    Authors: Lu Wang, Yan Luo, Liping Luo, Dandan Wu, Xiaofeng Ding, Handong Zheng et al.
    Journal of Experimental Medicine

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