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Mouse VEGF DuoSet ELISA

Catalog #: DY493
58 Citations 3 Reviews Datasheet / COA / SDS

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DY493 has been discontinued.
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Mouse VEGF ELISA Standard Curve
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse VEGF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse VEGF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse VEGF ELISA Standard Curve View Larger

Mouse VEGF ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and in adults (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues in a cystine knot structure and the formation of anti-parallel disulfide-linked dimers (4). Alternately spliced isoforms of 120, 164 and 188 amino acids (aa) have been found in mice, while 121, 145, 165, 183, 189, and 206 aa isoforms have been identified in humans (2, 4). In humans, VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). The same pattern may exist in mice. Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Mouse VEGF164 shares 97% aa sequence identity with corresponding regions of rat VEGF. It also shares 89% aa sequence identity with human and porcine VEGF, 88% with bovine VEGF, and 90% with feline, equine, and canine VEGF. VEGF is expressed in multiple cells and tissues including skeletal and cardiac muscle (5, 6), hepatocytes (7), osteoblasts (8), neutrophils (9), macrophages (10), keratinocytes (11), brown adipose tissue (12), CD34+ stem cells (13), endothelial cells (14), fibroblasts, and vascular smooth muscle cells (15). VEGF expression is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M, and TNF-alpha (3, 4, 9, 16). The isoforms are differentially expressed during development and in the adult (3). 

VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR), and induce their homodimerization and autophosphorylation (3, 4, 7, 17, 18). These receptors have seven extracellular immunoglobulin-like domains and an intracellular split tyrosine kinase domain. They are expressed on vascular endothelial cells and a range of non-endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 also binds the semaphorin receptor, neuropilin-1, which promotes complex formation with VEGF R2 (19). 
VEGF is best known for its role in vasculogenesis. During embryogenesis, VEGF regulates the proliferation, migration, and survival of endothelial cells (3, 4), thus regulating blood vessel density and size but playing no role in determining vascular patterns. VEGF promotes bone formation through osteoblast and chondroblast recruitment and is also a monocyte chemoattractant (20-22). In postnatal life, VEGF maintains endothelial cell integrity and is a potent mitogen for micro- and macro-vascular endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). In diseased tissues, VEGF promotes vascular permeability. It is thus thought to contribute to tumor metastasis by promoting both extravasation and tumor angiogenesis (23, 24). Various strategies have been employed therapeutically to antagonize VEGF-mediated tumor angiogenesis (25). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus (26).

Long Name:
Vascular Endothelial Growth Factor
Entrez Gene IDs:
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names:
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse VEGF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

58 Citations: Showing 1 - 10
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  1. Knocking-Down CD147/EMMPRIN Expression in CT26 Colon Carcinoma Forces the Cells into Cellular and Angiogenic Dormancy That Can Be Reversed by Interactions with Macrophages
    Authors: G Feigelman, E Simanovich, P Brockmeyer, MA Rahat
    Biomedicines, 2023-03-02;11(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Finerenone, a Non-Steroidal Mineralocorticoid Receptor Antagonist, Reduces Vascular Injury and Increases Regulatory T-Cells: Studies in Rodents with Diabetic and Neovascular Retinopathy
    Authors: JR Jerome, D Deliyanti, V Suphapimol, P Kolkhof, JL Wilkinson-
    International Journal of Molecular Sciences, 2023-01-25;24(3):.
    Species: Mouse
    Sample Types: Cell Lysates
  3. Changes in adenosine receptors and neurotrophic factors in the SOD1G93A mouse model of amyotrophic lateral sclerosis: Modulation by chronic caffeine
    Authors: N Rei, CA Valente, SH Vaz, M Farinha-Fe, JA Ribeiro, AM Sebastião
    PLoS ONE, 2022-12-14;17(12):e0272104.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. KDM6A Loss Recruits Tumor-Associated Neutrophils and Promotes Neutrophil Extracellular Trap Formation in Pancreatic Cancer
    Authors: J Yang, L Jin, HS Kim, F Tian, Z Yi, K Bedi, M Ljungman, M Pasca di M, H Crawford, J Shi
    Cancer Research, 2022-11-15;0(0):OF1-OF14.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis
    Authors: A Scanu, R Luisetto, F Oliviero, F Galuppini, V Lazzarin, G Pennelli, S Masiero, L Punzi
    International Journal of Molecular Sciences, 2022-10-28;23(21):.
    Species: Mouse
    Sample Types: Serum
  6. Mild dyslipidemia accelerates tumorigenesis through expansion of Ly6Chi monocytes and differentiation to pro-angiogenic myeloid cells
    Authors: T Tran, JR Lavillegra, C Lereverend, B Esposito, L Cartier, M Montabord, J Tran-Rajau, M Diedisheim, N Gruel, K Ouguerram, L Paolini, O Lenoir, E Pinteaux, E Brabencova, C Tanchot, P Urquia, J Lehmann-Ch, R Le Naour, Y Merrouche, C Stockmann, Z Mallat, A Tedgui, H Ait-Oufell, E Tartour, S Potteaux
    Nature Communications, 2022-09-14;13(1):5399.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Complement activation contributes to subretinal fibrosis through the induction of epithelial-to-mesenchymal transition (EMT) in retinal pigment epithelial cells
    Authors: M Llorián-Sa, EM Byrne, M Szczepan, K Little, M Chen, H Xu
    Journal of Neuroinflammation, 2022-07-14;19(1):182.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. VEGF-Mediated Augmentation of Autophagic and Lysosomal Activity in Endothelial Cells Defends against Intracellular Streptococcus pyogenes
    Authors: SL Lu, H Omori, Y Zhou, YS Lin, CC Liu, JJ Wu, T Noda
    MBio, 2022-07-05;0(0):e0123322.
    Species: Mouse
    Sample Types: Serum
  9. Sirtuin 1 Induces Choroidal Neovascularization and Triggers Age-Related Macular Degeneration by Promoting LCN2 through SOX9 Deacetylation
    Authors: S Zhao, Z Huang, H Jiang, J Xiu, L Zhang, Q Long, Y Yang, L Yu, L Lu, H Gu
    Oncogene, 2022-06-09;2022(0):1671438.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Association between vascular endothelial growth factor-mediated blood-brain barrier dysfunction and stress-induced depression
    Authors: H Matsuno, S Tsuchimine, K O'Hashi, K Sakai, K Hattori, S Hidese, S Nakajima, S Chiba, A Yoshimura, N Fukuzato, M Kando, M Tatsumi, S Ogawa, N Ichinohe, H Kunugi, K Sohya
    Molecular Psychiatry, 2022-05-26;0(0):.
    Species: Mouse
    Sample Types: Serum
  11. Kinetics of LYVE-1-positive M2-like macrophages in developing and repairing dental pulp in vivo and their pro-angiogenic activity in vitro
    Authors: TQ Kieu, K Tazawa, N Kawashima, S Noda, M Fujii, K Nara, K Hashimoto, P Han, T Okiji
    Scientific Reports, 2022-03-25;12(1):5176.
    Species: Mouse
    Sample Types: Conditioned Media
  12. Macrophage IL-1beta promotes arteriogenesis by autocrine STAT3- and NF-kappaB-mediated transcription of pro-angiogenic VEGF-A
    Authors: CS Mantsounga, C Lee, J Neverson, S Sharma, A Healy, JM Berus, C Parry, NM Ceneri, F López-Girá, HJ Chun, Q Lu, F Sellke, G Choudhary, AR Morrison
    Cell Reports, 2022-02-01;38(5):110309.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Therapeutic Effects of Fenofibrate Nano-Emulsion Eye Drops on Retinal Vascular Leakage and Neovascularization
    Authors: L Huang, W Liang, K Zhou, RA Wassel, ZD Ridge, JX Ma, B Wang
    Biology, 2021-12-15;10(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Intramuscular Exposure to a Lethal Dose of Ricin Toxin Leads to Endothelial Glycocalyx Shedding and Microvascular Flow Abnormality in Mice and Swine
    Authors: A Sapoznikov, Y Gal, Y Evgy, M Aftalion, S Katalan, T Sabo, C Kronman, R Falach
    International Journal of Molecular Sciences, 2021-11-16;22(22):.
    Species: Mouse
    Sample Types: Serum
  15. Topical administration of the kappa opioid receptor agonist nalfurafine suppresses corneal neovascularization and inflammation
    Authors: H Shokirova, T Inomata, T Saitoh, J Zhu, K Fujio, Y Okumura, A Yanagawa, K Fujimoto, J Sung, A Eguchi, M Miura, K Nagino, K Hirosawa, M Kuwahara, Y Akasaki, H Nagase, A Murakami
    Scientific Reports, 2021-04-21;11(1):8647.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes
    Authors: D Philipp, M Holthaus, V Basoah, K Pfannkuche, L Suhr, T Wahlers, A Paunel-Gör
    Stem Cells International, 2021-04-10;2021(0):8888575.
    Species: Mouse
    Sample Types: Cell Lysates
  17. Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke
    Authors: LR Lemmerman, MHH Balch, JT Moore, D Alzate-Cor, MA Rincon-Ben, A Salazar-Pu, S Gnyawali, HN Harris, W Lawrence, L Ortega-Pin, L Wilch, IB Risser, AJ Maxwell, S Duarte-San, D Dodd, GP Guio-Vega, DM McTigue, WD Arnold, SM Nimjee, CK Sen, S Khanna, C Rink, N Higuita-Ca, D Gallego-Pe
    Science Advances, 2021-03-19;7(12):.
    Species: Mouse
    Sample Types: Cell Lysates
  18. The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
    Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
    Embo Molecular Medicine, 2021-03-10;0(0):e13162.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Inhibitory effect of ginsenoside Rg3 on cancer stemness and mesenchymal transition in breast cancer via regulation of myeloid-derived suppressor cells
    Authors: JH Song, DY Eum, SY Park, YH Jin, JW Shim, SJ Park, MY Kim, SJ Park, K Heo, YJ Choi
    PLoS ONE, 2020-10-22;15(10):e0240533.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Recombinant Myxoma Virus-Derived Immune Modulator M-T7 Accelerates Cutaneous Wound Healing and Improves Tissue Remodeling
    Authors: JR Yaron, L Zhang, Q Guo, EA Awo, M Burgin, LN Schutz, N Zhang, J Kilbourne, J Daggett-Vo, KM Lowe, AR Lucas
    Pharmaceutics, 2020-10-22;12(11):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Ischemia-Like Stress Conditions Stimulate Trophic Activities of Adipose-Derived Stromal/Stem Cells
    Authors: J Bachmann, E Ehlert, M Becker, C Otto, K Radeloff, T Blunk, P Bauer-Krei
    Cells, 2020-08-21;9(9):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye
    Authors: A Wolf, M Herb, M Schramm, T Langmann
    Nat Commun, 2020-06-01;11(1):2709.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages
    Authors: YR Na, JW Kwon, DY Kim, H Chung, J Song, D Jung, H Quan, D Kim, JS Kim, YW Ju, W Han, HS Ryu, YS Lee, JJ Hong, SH Seok
    Cell Rep, 2020-05-12;31(6):107643.
    Species: Mouse
    Sample Types:
  24. Functional Evaluation of AMD-Associated Risk Variants of Complement Factor B
    Authors: C Pilotti, J Greenwood, SE Moss
    Invest. Ophthalmol. Vis. Sci., 2020-05-11;61(5):19.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Different Frequency of Cyclic Tensile Strain Relates to Anabolic/Catabolic Conditions Consistent with Immunohistochemical Staining Intensity in Tenocytes
    Authors: Y Kubo, B Hoffmann, K Goltz, U Schnakenbe, H Jahr, R Merkel, G Schulze-Ta, T Pufe, M Tohidnezha
    Int J Mol Sci, 2020-02-06;21(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Distinct effects of general anesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models
    Authors: R Li, Y Huang, J Lin
    Nat Commun, 2020-01-31;11(1):642.
    Species: Mouse
    Sample Types: Serum
  27. ACE2 activation protects against cognitive decline and reduces amyloid pathology in the Tg2576 mouse model of Alzheimer's disease.
    Authors: Evans C, Miners J, Piva G, Willis C, Heard D, Kidd E, Good M, Kehoe P
    Acta Neuropathol, 2020-01-25;139(3):485-502.
    Species: Mouse
    Sample Types: Cell Lysates
  28. Inhibition of FLT1 ameliorates muscular dystrophy phenotype by increased vasculature in a mouse model of Duchenne muscular dystrophy
    Authors: M Verma, Y Shimizu-Mo, Y Asakura, JP Ennen, J Bosco, Z Zhou, GH Fong, S Josiah, D Keefe, A Asakura
    PLoS Genet., 2019-12-26;15(12):e1008468.
    Species: Mouse
    Sample Types: Cell Culture Su
  29. A Virus-Derived Immune Modulating Serpin Accelerates Wound Closure with Improved Collagen Remodeling
    Authors: L Zhang, JR Yaron, AM Tafoya, SE Wallace, J Kilbourne, S Haydel, K Rege, G McFadden, AR Lucas
    J Clin Med, 2019-10-04;8(10):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. Oral administration of EPA-rich oil impairs collagen reorganization due to elevated production of IL-10 during skin wound healing in mice
    Authors: B Burger, CMC Kühl, T Candreva, RDS Cardoso, JR Silva, BG Castelucci, SR Consonni, HL Fisk, PC Calder, MAR Vinolo, HG Rodrigues
    Sci Rep, 2019-06-24;9(1):9119.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling
    Authors: M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura
    Cell Stem Cell, 2018-10-04;23(4):530-543.e9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. SOCS3 deficiency in myeloid cells promotes retinal degeneration and angiogenesis through arginase-1 up-regulation in experimental autoimmune uveoretinitis
    Authors: M Chen, J Zhao, IH Ali, S Marry, J Augustine, M Bhuckory, A Lynch, A Kissenpfen, H Xu
    Am. J. Pathol., 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. Proangiogenic alginate-g-pyrrole hydrogel with decoupled control of mechanical rigidity and electrically conductivity
    Authors: RJ DeVolder, Y Seo, H Kong
    Biomater Res, 2017-11-07;21(0):24.
    Species: Mouse
    Sample Types: Whole Cells
  34. MCPIP1 downregulation in clear cell renal cell carcinoma promotes vascularization and metastatic progression
    Authors: P Marona, J Górka, Z Mazurek, W Wilk, J Rys, M Majka, J Jura, K Miekus
    Cancer Res., 2017-07-17;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. ECM-based macroporous sponges release essential factors to support the growth of hematopoietic cells
    J Control Release, 2016-09-23;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Interactions among Lung Cancer Cells, Fibroblasts, and Macrophages in 3D Co-Cultures and the Impact on MMP-1 and VEGF Expression
    Authors: Xiao-Qing Liu
    PLoS ONE, 2016-05-27;11(5):e0156268.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Tumor Microenvironment Remodeling by 4-Methylumbelliferone Boosts the Antitumor Effect of Combined Immunotherapy in Murine Colorectal Carcinoma.
    Authors: Malvicini M, Fiore E, Ghiaccio V, Piccioni F, Rizzo M, Olmedo Bonadeo L, Garcia M, Rodriguez M, Bayo J, Peixoto E, Atorrasagasti C, Alaniz L, Aquino J, Matar P, Mazzolini G
    Mol Ther, 2015-06-24;23(9):1444-55.
    Species: Mouse
    Sample Types: Serum
  38. VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.
    Authors: Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, Latreche S, Bergaya S, Benhamouda N, Tanchot C, Stockmann C, Combe P, Berger A, Zinzindohoue F, Yagita H, Tartour E, Taieb J, Terme M
    J Exp Med, 2015-01-19;212(2):139-48.
    Species: Mouse
    Sample Types: Tissue Homogenates
  39. FcgammaRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis.
    Authors: Clatworthy M, Harford S, Mathews R, Smith K
    Proc Natl Acad Sci U S A, 2014-12-04;111(50):17971-6.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2014-10-22;515(7525):130-3.
    Species: Mouse
    Sample Types: Tissue Homogenates
  41. Role of TGF-beta signaling in generation of CD39+CD73+ myeloid cells in tumors.
    Authors: Ryzhov S, Pickup M, Chytil A, Gorska A, Zhang Q, Owens P, Feoktistov I, Moses H, Novitskiy S
    J Immunol, 2014-08-15;193(6):3155-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Advances in the pathophysiology of pre-eclampsia and related podocyte injury.
    Authors: Craici, Iasmina, Wagner, Steven J, Weissgerber, Tracey L, Grande, Joseph P, Garovic, Vesna D
    Kidney Int, 2014-02-26;86(2):275-85.
    Species: Rat
    Sample Types:
  43. The monocyte to macrophage transition in the murine sterile wound.
    Authors: Crane, Meredith, Daley, Jean M, van Houtte, Olivier, Brancato, Samielle, Henry, William, Albina, Jorge E
    PLoS ONE, 2014-01-22;9(1):e86660.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  44. A2B adenosine receptors prevent insulin resistance by inhibiting adipose tissue inflammation via maintaining alternative macrophage activation.
    Authors: Csoka B, Koscso B, Toro G, Kokai E, Virag L, Nemeth Z, Pacher P, Bai P, Hasko G
    Diabetes, 2013-11-05;63(3):850-66.
    Species: Mouse
    Sample Types: Plasma
  45. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013-03-08;190(7):3500-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development.
    Authors: Lange CA, Luhmann UF, Mowat FM
    Development, 2012-05-23;139(13):2340-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
  47. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Ascites Fluid
  48. Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex.
    Authors: Howell KR, Kutiyanawalla A, Pillai A
    PLoS ONE, 2011-05-27;6(5):e20198.
    Species: Mouse
    Sample Types: Serum
  49. CD11c(hi) dendritic cells regulate the re-establishment of vascular quiescence and stabilization after immune stimulation of lymph nodes.
    Authors: Tzeng TC, Chyou S, Tian S, Webster B, Carpenter AC, Guaiquil VH, Lu TT
    J. Immunol., 2010-03-15;184(8):4247-57.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. IL-6 loss causes ventricular dysfunction, fibrosis, reduced capillary density, and dramatically alters the cell populations of the developing and adult heart.
    Authors: Banerjee I, Fuseler JW, Intwala AR, Baudino TA
    Am. J. Physiol. Heart Circ. Physiol., 2009-02-20;296(5):H1694-704.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
    Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2008-12-18;41(1):59-68.
    Species: Mouse
    Sample Types: Tissue Homogenates
  52. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
    Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
    J. Immunol., 2008-11-01;181(9):6384-93.
    Species: Mouse
    Sample Types: Tissue Homogenates
  53. Fibroblast-type reticular stromal cells regulate the lymph node vasculature.
    Authors: Chyou S, Ekland EH, Carpenter AC, Tzeng TC, Tian S, Michaud M, Madri JA, Lu TT
    J. Immunol., 2008-09-15;181(6):3887-96.
    Species: Mouse
    Sample Types: Tissue Homogenates
  54. Adenosine receptors in regulation of dendritic cell differentiation and function.
    Authors: Novitskiy SV, Ryzhov S, Zaynagetdinov R, Goldstein AE, Huang Y, Tikhomirov OY, Blackburn MR, Biaggioni I, Carbone DP, Feoktistov I, Dikov MM
    Blood, 2008-06-17;112(5):1822-31.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. In the adult mesenchymal stem cell population, source gender is a biologically relevant aspect of protective power.
    Authors: Crisostomo PR, Markel TA, Wang M, Lahm T, Lillemoe KD, Meldrum DR
    Surgery, 2007-08-01;142(2):215-21.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. A functional comparison of canine and murine bone marrow derived cultured mast cells.
    Authors: Lin TY, London CA
    Vet. Immunol. Immunopathol., 2006-10-06;114(3):320-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. Impaired inflammatory angiogenesis, but not leukocyte influx, in mice lacking TNFR1.
    Authors: Barcelos LS, Talvani A, Teixeira AS, Vieira LQ, Cassali GD, Andrade SP, Teixeira MM
    J. Leukoc. Biol., 2005-05-13;78(2):352-8.
    Species: Mouse
    Sample Types: Complex Sample Type
  58. Short-term BMP-2 expression is sufficient for in vivo osteochondral differentiation of mesenchymal stem cells.
    Authors: Noel D, Gazit D, Bouquet C, Apparailly F, Bony C, Plence P, Millet V, Turgeman G, Perricaudet M, Sany J, Jorgensen C
    Stem Cells, 2004-01-01;22(1):74-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse VEGF DuoSet ELISA
By Jordi Lambert on 03/29/2018
Sample Tested: mouse cell conditioned media

Worked perfectly for standards, my samples and a control of diluted recombinant VEGF. Excellent elisa and easy to use

Mouse VEGF DuoSet ELISA DY493
Mouse VEGF DuoSet ELISA
By Chinmay Surve on 05/17/2017
Mouse VEGF DuoSet ELISA
By Anonymous on 05/02/2017
Sample Tested: Cell culture supernatant