Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes. This protocol describes a technique to promote the osteogenic differentiation of human and mouse MSCs.
Please read the protocol in its entirety before starting.
Note: Sterile technique is required when handling the reagents.
Figure 1
Note: When handling biohazard materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Figure 2. Osteocyte Differentiation of Human MSCs. Human mesenchymal stem cells differentiated in vitro for 21 days using StemXVivo Human/Mouse Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Human StemXVivo Osteogenic Supplement (Catalog # CCM008) were stained for the osteogenic lineage marker, Osteocalcin using a Mouse Anti-Human/Rat Osteocalcin Monoclonal Antibody (Catalog # MAB1419) and NorthernLights 557-conjugated Anti-mouse Secondary Antibody (Catalog # NL007; red). Cells were counterstained with DAPI (blue). | Figure 3. Osteocyte Differentiation of Mouse MSCs. The Mouse Mesenchymal Stem Cell Functional Identification Kit (Catalog # SC010) was used to differentiate MSCs into the osteogenic lineage. Mature differentiated cells were stained for the osteogenic lineage marker Osteopontin supplied with the kit (red). The nuclei were counterstained with DAPI (blue). Similar results may be obtained using the StemXVivo Human/Mouse Osteogenic/Adipogenic Base Media (Catalog # CCM007) and Mouse StemXVivo Osteogenic Supplement (Catalog # CCM009). |