"DNA" has 165 results in Resources.

Eukaryotic and prokaryotic cells possess multiple mechanisms to repair DNA and control damage to their genomes. These include base excision repair (BER) and nucleotide excision repair (NER) that excise and replace damaged nucleotide bases and helix-distorting lesions, respectively. Many of the proteins involved in NER are also active in the related transcription-coupled repair (TCR). In...

DNA Damage Repair Enzymes are necessary for the repair of errors introduced during DNA replication or recombination or as a result of treatment with exogenous genotoxic agents. These enzymes can be used in vitro to detect specific forms of DNA damage or for repair assays. The DNA damage repair substrates are oligonucleotides that contain specific mutations which are recognized and cleaved by some...

First Printed in R&D Systems 2003 Catalog Contents Introduction Cell Cycle Checkpoints G1 Checkpoint S-phase Checkpoint G2 Checkpoint DNA Repair Pathways References Introduction The maintenance of genome integrity and fidelity is essential for the proper function and survival of all organisms. This task is particularly daunting due to constant assault on the DNA by genotoxic...

Cellular senescence and organismal aging are both accompanied by increased DNA damage. Furthermore, damaged DNA has been shown to accumulate during aging in many tissues, including brain, muscle, kidney and liver. It remains to be determined if DNA damage is purely a result of the aging process or part of the cause. Several studies have shown that dietary (calorie) restriction reduces the amount...

Eukaryotic and prokaryotic cells possess multiple mechanisms to repair DNA and control damage to their genomes. These include base excision repair (BER) and nucleotide excision repair (NER) that excise and replace damaged nucleotide bases and helix-distorting lesions, respectively. Many of the enzymes involved in NER are also active in transcription-coupled repair (TCR) processes. In addition,...

In addition to base and nucleotide excision repair molecules, mismatch repair (MMR) proteins act to replace mismatched nucleotides and repair insertion/deletion loops. Genotoxic stress can introduce DNA double-strand breaks (DSBs), which are repaired by either homologous recombination or non-homologous end-joining. The Mre11/Rad50/Nbs1 (MRN) complex, along with members of the Rad51 family of...

Highly purified and qualified for Northern, Southern, and dot blotting procedures. Purified DNA is used as a blocking agent in pre-hybridization and hybridization to prevent non-specific binding. DNA is provided at a concentration of 10 mg/mL which allows convenient addition to buffers to create a final recommended working concentration of 0.1 mg/mL with a fragment size range of 200 - 500 base...

Download DNA-Damage Poster The human genome is exposed to potentially deleterious genotoxic events during every cell division cycle. This endogenous source of DNA damage results from cellular metabolism or routine errors in DNA replication and recombination. In addition, cellular and organismal exposure to exogenous genotoxic agents such as ionizing radiation, ultraviolet light, oxidative...

DNA Damage Detection CometAssay® Enzyme Activity Assays PARP Universal Colorimetric Assay Additional Genotoxic Stress-related Products CometAssay® Single Cell Gel Electrophoresis Assay Catalog #: 4250-050-K, 4251-050-KLabel: SYBR Green Dye Silver StainDescription: Cells are immobilized on a slide in a bed of low melting point agarose and then gently lysed. Following...

Click for larger image. The human genome is exposed to potentially deleterious events during every cell division cycle. Cellular metabolism or routine errors in DNA replication and recombination represent endogenous sources of potential DNA damage. In addition, exposure to exogenous genotoxic agents such as ionizing radiation (IR), ultraviolet light (UV), oxidative stress, and chemical mutagens...