"Primary" has 230 results in Resources.
Primary Cells
Product CategoriesRat Cortical Stem Cells Rat Cortical Stem Cells (Catalog # NSC001) Rat Cortical Stem Cell Culture Protocol Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems now offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. Every lot of R&D Systems Rat Cortical Stem Cells...
More InfoConjugated Primary Antibodies
Product CategoriesR&D Systems offers a wide variety of conjugated primary antibodies allowing you to analyze multiple targets with ease. Our extensive selection of conjugated antibodies in a variety of fluorescent and enzymatic labels allows you to customize your experiment. We offer bright conjugates for low abundance analytes and subdued dyes for high abundance targets. Find your antibody panel today! See...
More InfoPrimary Antibody Selection & Optimization
ProtocolsThe most important factor when designing an IHC/ICC experiment is selection of the primary antibody. In turn, the critical feature of a primary antibody is specificity for the epitope. All steps of an IHC/ICC experiment must be optimized to visualize specific staining and minimize non-specific background signals. This includes performing initial studies to determine the appropriate incubation...
More InfoPrimary Mouse Cortical Stem Cells
Product CategoriesMouse Cortical Stem Cells (Catalog # NSC002) Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 CD-1 mice. These cells are designated as passage 1 (P1) cells. Cells were cultured as a monolayer, passaged once, and the expanded progeny...
More InfoPrimary Rat Cortical Stem Cells
Product CategoriesRat Cortical Stem Cells (Catalog # NSC001) Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. These cells are designated as passage 0 (P0) cells. Cells were cultured as a monolayer system. After 5 days the cells were...
More InfoPrimary Mouse & Rat Cortical Stem Cells
Product CategoriesFor cortical stem cell expansion and differentiation into neurons, astrocytes, and oligodendrocytes. Product Summary Neural stem cells expanded ex vivo serve as excellent tools for researchers studying neural development and neurological disorders. Every lot of R&D Systems Mouse and Rat Cortical Stem Cells is tested for a high level of Nestin expression and the capacity for multi-lineage...
More InfoIsolation and Culture of Primary Cells from Bone Marrow
BlogPaul Maye, Ph.D., Assistant Professor, University of Connecticut Health Center Bone marrow stromal cells have been one of the more intensively studied adult stem cell types over the past 50 years. There is great interest within regenerative medicine and wound healing for this adult stem cell type because of its complex biological properties including its ability to support a hematopoietic...
More InfoIL-15 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
PathwaysInterleukin-15 (IL-15) is a four alpha-helix bundle cytokine that is structurally and functionally related to IL-2. It is produced primarily by dendritic cells, monocytes, and epithelial cells. The heterotrimeric IL-15 receptor complex consists of a unique IL-15 R alpha subunit, IL-2/IL-15 R beta, and the common gamma-chain/IL-2 R gamma subunit. Unlike IL-2, IL-15 binds with high affinity to...
More InfoIL-2 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
PathwaysInterleukin-2 (IL-2) is an O-glycosylated four alpha-helix bundle cytokine that is primarily produced by activated T cells, dendritic cells, and B cells. The biological activity of IL-2 is mediated by binding to a cell surface receptor complex consisting of IL-2 R alpha/CD25, IL-2/IL-15 R beta, and the common gamma-chain/IL-2 R gamma subunit. IL-2 can also bind with low affinity to IL-2 R alpha...
More InfoIL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
PathwaysInterleukin-21 (IL-21) is the most recently described cytokine belonging to the common cytokine receptor gamma-chain family. Like other common gamma chain family members, IL-21 is a four alpha-helix bundle type I cytokine. It signals through a receptor complex consisting of IL-21 R and common gamma-chain/IL-2 R gamma. IL-21 is produced primarily by CD4+ T cells and natural killer T (NKT) cells...
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