Get the most out of your primary antibodies!
Designed to optimize staining on cryostat, paraffin-embedded, and free-floating tissue sections, these kits provide all the reagents required to maximize your IHC experiments.
Key Benefits
- Ready to use
- High Sensitivity Streptavidin (HSS) increases detection sensitivity
- Suitable for tissue sections & cytospin preparations
- Pre-diluted reagents minimize hands-on time
- Optimizes mouse, rabbit, or goat IgG primary antibody staining
Kit Components for IHC Optimization
Blocking reagents |
To minimize non-specific Peroxidase, Avidin, and Biotin signals |
Secondary antibodies |
Biotinylated and pre-diluted to minimize hands-on time |
HSS-conjugated HRP |
Chemically optimized Streptavidin displays little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins |
Chromogen |
Choose DAB (brown) or AEC (red) |
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Cell & Tissue Staining Kits Overview
Intended for the localization of antigens in a broad range of histological and cytological specimens, these kits may be used with primary IgG antibodies manufacturd by R&D System or by other vendors. Briefly, detection is based on the formation of the Avidin-Biotin Complex (ABC). Visualization is based on enzymatic conversion of chromogenic substrates 3,3’ Diaminobenzidine (DAB) or 3-amino-9-ethylcarbazole (AEC) into a colored precipitate (brown or red, respectively), by horseradish peroxidase (HRP) at the sites of antigen localization, which can then be viewed using bright-field microscopy.
The high detection sensitivity of R&D Systems Cell and Tissue Staining Kits is achieved by using premium quality biotinylated secondary antibodies and High Sensitivity Streptavidin-conjugated HRP (HSS-HRP). HSS is a chemical analog of Streptavidin that has little net positive charge at neutral or slightly alkaline pH and will interact only with Biotin attached to secondary antibodies. HSS-HRP shows little or no non-specific binding to phospholipids, nucleic acids, and carbohydrate binding proteins.
The LSAB Method of IHC Signal Amplification. When using biotinylated secondary antibodies, further amplification of the signal can be achieved by taking advantage of the strong affinity of Avidin and Streptavidin for Biotin. Avidin is a glycoprotein in egg white that combines stoichiometrically with Biotin. Streptavidin is purified from the bacterium Streptomyces avidinii, is not glycosylated, and exhibits lower non-specific binding than Avidin. Both proteins bind four Biotins per molecule. If a biotinylated secondary antibody is employed, the signal can be significantly amplified by subsequent incubation with labeled streptavidin-biotin (LSAB Method). Streptavidin may be conjugated to a detection enzyme (e.g. horseradish peroxidase or alkaline phosphatase) or a fluorochrome. Using this amplification method requires additional steps to prevent non-specific binding of Avidin and Biotin. All required reagents are included in our Cell & Tissue Staining Kits.