R&D Systems offers a range of high quality products for IHC/ICC experiments. Our primary antibodies are manufactured under controlled conditions to ensure lot-to-lot consistency, high specificity, and outstanding performance. The NorthernLights™ range of fluorescent secondary antibodies and streptavidin conjugates are bright, resistant to photobleaching and are ideal for multi-color fluorescence microscopy. In addition, we offer complete Cell & Tissue Staining Kits which contain all the materials required for visualization based on enzymatic conversion of the chromogenic substrates DAB and AEC.
The most challenging aspect of the IHC/ICC technique is determining the precise experimental conditions required to generate a strong and specific signal for each antigen of interest. To facilitate successful IHC/ICC experiments, R&D Systems provides specific protocols for staining of cells and tissues. For additional support, please refer to our troubleshooting guide, IHC/ICC image gallery, and other related resources.
IHC/ICC Protocols
- Making a 4% Formaldehyde Solution in PBS
- Gelatin-coated Slides for Histological Tissue Sections
- Fluorescent ICC Staining of Cells on Coverslips
- Fluorescent ICC Staining of Stem Cells on Coverslips
- Fluorescent ICC Staining of Non-adherent Cells
- Chromogenic IHC Staining of Paraffin-embedded Tissue Section
- Chromogenic IHC Staining of Frozen Tissue Sections
- Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Fluorescent IHC Staining of Frozen Tissue Sections
- Heat-induced Epitope Retrieval (HIER)
Further IHC/ICC Support
- Designing a Successful IHC/ICC Experiment
- Preparing Samples for IHC/ICC Experiments
- Appropriate Fixation of IHC/ICC Samples
- Preventing Non-specific Staining
- Primary Antibody Selection & Optimization
- Detection & Visualization of Antibody Binding
- The Importance of IHC/ICC Controls
- Antigen Retrieval Methods
- Troubleshooting IHC/ICC Experiments
- IHC/ICC Supplementary Reagents
- Download complimentary IHC Screensavers