R&D Systems offers reagents to promote epitope availability and enhance immunoreactivity. VisUCyte Antigen Retrieval Reagent-Basic* (Catalog # VCTS021), Antigen Retrieval Reagent-Acidic* (Catalog # VCTS022) and Antigen Retrieval Reagent-Universal (Catalog # VCTS023) are three different antigen retrieval systems that utilize heat-induced recovery of cell and tissue antigens. Each reagent is supplied as a 10X concentrate (50 mL).
*Unlike Universal solution, Acidic and Basic solutions may also affect tissue morphology. The optimal antigen unmasking condition is a function of temperature (90 - 100° C) and incubation time (up to 30 minutes) and should be established by each individual investigator.