Home / Adiponectin/Acrp30 / Human Adiponectin/Acrp30 DuoSet ELISA

Human Adiponectin/Acrp30 DuoSet ELISA

Catalog #: DY1065
68 Citations 7 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1065
DY1065-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Adiponectin / Acrp30 ELISA Standard Curve
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Product Details
Procedure
Citations (68)
FAQs
Supplemental Products
Reviews (7)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Adiponectin/Acrp30 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Adiponectin / Acrp30. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent: 1% BSA in PBS, pH 7.2 - 7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer: 1% BSA in PBS, pH 7.2 - 7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

 

Scientific Data

N/A Human Adiponectin / Acrp30 ELISA Standard Curve View Larger

Human Adiponectin / Acrp30 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody (to the working concentration stated in the product datasheet ) in PBS without carrier protein. Immediately coat a 96-well microplate with 100 µL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 µL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block each well of the microplate as recommended in the product datasheet. Incubate at room temperature for a minimum of 1 hour.

    Note: The recommended Reagent Diluent typically contains 1% BSA. Some DuoSet Development Kits require alternative blocking agents, or for plates to be blocked overnight with a higher percentage of BSA, please see the product datasheet for details.
     
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

PRECAUTION
The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Assay Procedure

  1. Add 100 µL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 µL of the Detection Antibody, diluted in Reagent Diluent (as recommended in the product datasheet), to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 µL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 µL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 µL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human Adiponectin/Acrp30 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

68 Citations: Showing 1 - 10
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  1. Cognitive function is associated with performance in time up and go test and with leptin blood levels in community-dwelling older women
    Authors: da Costa Teixeira, LA;Soares, LA;Lima, LP;Avelar, NCP;de Moura, JA;Leopoldino, AAO;Figueiredo, PHS;Parentoni, AN;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  2. Trauma-associated extracellular histones mediate inflammation via a MYD88-IRAK1-ERK signaling axis and induce lytic cell death in human adipocytes
    Authors: Roos, J;Zinngrebe, J;Huber-Lang, M;Lupu, L;Schmidt, MA;Strobel, H;Westhoff, MA;Stifel, U;Gebhard, F;Wabitsch, M;Mollnes, TE;Debatin, KM;Halbgebauer, R;Fischer-Posovszky, P;
    Cell death & disease
    Species: Human
    Sample Types: Cell Culture Supernates
  3. The strong inverse association between plasma concentrations of soluble tumor necrosis factor receptors type 1 with adiponectin/leptin ratio in older women
    Authors: Augusto da Costa Teixeira, L;Rocha-Vieira, E;Aparecida Soares, L;Mota de Oliveira, F;Aparecida Oliveira Leopoldino, A;Netto Parentoni, A;Amaral Mendonça, V;Cristina Rodrigues Lacerda, A;
    Cytokine
    Species: Human
    Sample Types: Plasma
  4. Circulating Adipocytokines and Insulin Like-Growth Factors and Their Modulation in Obesity-Associated Endometrial Cancer
    Authors: Ray, I;Möller-Levet, CS;Michael, A;Butler-Manuel, S;Chatterjee, J;Tailor, A;Ellis, PE;Meira, LB;
    Cancers
    Species: Human
    Sample Types: Plasma
  5. Relationships between Circulating Biomarkers and Body Composition Parameters in Patients with Metabolic Syndrome: A Community-Based Study
    Authors: Tarabeih, N;Kalinkovich, A;Ashkenazi, S;Cherny, SS;Shalata, A;Livshits, G;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  6. Extracellular vesicles are carriers of adiponectin with insulin-sensitizing and anti-inflammatory properties
    Authors: Blandin, A;Amosse, J;Froger, J;Hilairet, G;Durcin, M;Fizanne, L;Ghesquière, V;Prieur, X;Chaigneau, J;Vergori, L;Dray, C;Pradère, JP;Blandin, S;Dupont, J;Ducluzeau, PH;Dubois, S;Boursier, J;Cariou, B;Le Lay, S;
    Cell reports
    Species: Human
    Sample Types: Plasma
  7. Dysregulation of adipokines levels among healthy first-degree relatives of type 2 diabetes patients
    Authors: Purnamasari, D;Simanjuntak, CK;Tricaesario, C;Tahapary, DL;Harbuwono, DS;Yunir, E;
    Heliyon
    Species: Human
    Sample Types: Serum
  8. Effects of an Intervention with Selenium and Coenzyme Q10 on Five Selected Age-Related Biomarkers in Elderly Swedes Low in Selenium: Results That Point to an Anti-Ageing Effect-A Sub-Analysis of a Previous Prospective Double-Blind Placebo-Controlled Randomised Clinical Trial
    Authors: Alehagen, U;Alexander, J;Aaseth, JO;Larsson, A;Svensson, E;Opstad, TB;
    Cells
    Species: Human
    Sample Types: Plasma
  9. Inflammatory biomarkers at different stages of Sarcopenia in older women
    Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  10. Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators in overweight women suggest a state of subclinical endotoxemia
    Authors: Metz, CN;Xue, X;Chatterjee, PK;Adelson, R;Brines, M;Tracey, KJ;Gregersen, PK;Pavlov, VA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Plasma
  11. High Glucose Promotes Inflammation and Weakens Placental Defenses against E. coli and S. agalactiae Infection: Protective Role of Insulin and Metformin
    Authors: R Jiménez-Es, D Vargas-Alc, P Flores-Esp, AC Helguera-R, O Villavicen, I Mancilla-H, C Irles, YD Torres-Ram, MY Valdespino, P Velázquez-, R Zamora-Esc, M Islas-Lópe, C Carranco-S, L Díaz, V Zaga-Clave, A Olmos-Orti
    International Journal of Molecular Sciences, 2023-03-09;24(6):.
    Species: Human
    Sample Types: Placental Supernates
  12. Obesity promotes radioresistance through SERPINE1-mediated aggressiveness and DNA repair of triple-negative breast cancer
    Authors: YH Su, YZ Wu, DK Ann, JL Chen, CY Kuo
    Cell Death & Disease, 2023-01-21;14(1):53.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
    Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
    Journal of Clinical Medicine, 2022-12-02;11(23):.
    Species: Human
    Sample Types: Plasma
  14. Blood orange juice intake modulates plasma and PBMC microRNA expression in overweight and insulin resistance women: impact on MAPK and NFkappaB signaling pathways
    Authors: VC Capetini, BJ Quintanilh, DC de Oliveir, AH Nishioka, LA de Matos, LRP Ferreira, FM Ferreira, GR Sampaio, NMA Hassimotto, FM Lajolo, RA Fock, MM Rogero
    The Journal of nutritional biochemistry, 2022-11-26;0(0):109240.
    Species: Human
    Sample Types: Plasma
  15. Exercise training-induced changes in immunometabolic markers in youth badminton athletes
    Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
    Scientific Reports, 2022-09-15;12(1):15539.
    Species: Human
    Sample Types: Plasma
  16. Regulation of Adipose Progenitor Cell Expansion in a Novel Micro-Physiological Model of Human Adipose Tissue Mimicking Fibrotic and Pro-Inflammatory Microenvironments
    Authors: V Dani, S Bruni-Favi, B Chignon-Si, A Loubat, A Doglio, C Dani
    Cells, 2022-09-07;11(18):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Combined resistance and aerobic training improves lung function and mechanics and fibrotic biomarkers in overweight and obese women
    Authors: A Silva-Reis, MA Rodrigues, R Moraes-Fer, TG Gonçalves-, VH Souza-Palm, HC Aquino-San, ALL Bachi, LVF de Oliveir, RÁB Lopes-Mart, I Oliveira-S, R Albertini, CR Frison, RP Vieira
    Frontiers in Physiology, 2022-09-07;13(0):946402.
    Species: Human
    Sample Types: Plasma
  18. Di-(2-ethylhexyl) phthalate substitutes accelerate human adipogenesis through PPARgamma activation and cause oxidative stress and impaired metabolic homeostasis in mature adipocytes
    Authors: A Schaffert, I Karkossa, E Ueberham, R Schlichtin, K Walter, J Arnold, M Blüher, JT Heiker, J Lehmann, M Wabitsch, BI Escher, M von Bergen, K Schubert
    Environment international, 2022-05-06;164(0):107279.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. The effect of transdermal gender-affirming hormone therapy on markers of inflammation and hemostasis
    Authors: MH Schutte, R Kleemann, NM Nota, CM Wiepjes, JM Snabel, G T'Sjoen, A Thijs, M den Heijer
    PLoS ONE, 2022-03-15;17(3):e0261312.
    Species: Human
    Sample Types: Plasma
  20. Transcriptional and DNA Methylation Signatures of Subcutaneous Adipose Tissue and Adipose-Derived Stem Cells in PCOS Women
    Authors: A Divoux, E Erdos, K Whytock, TF Osborne, SR Smith
    Cells, 2022-03-01;11(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Pre-Transplant Serum Leptin Levels and Relapse of Acute Myeloid Leukemia after Allogeneic Transplantation
    Authors: MA Schwarzbic, H Dai, L Kordelas, DW Beelen, A Radujkovic, C Müller-Tid, P Dreger, T Luft
    International Journal of Molecular Sciences, 2022-02-20;23(4):.
    Species: Human
    Sample Types: Serum
  22. Transient Agarose Spot (TAS) Assay: A New Method to Investigate Cell Migration
    Authors: A Veres-Szék, D Pap, B Szebeni, L ?rfi, C Szász, C Pajtók, E Lévai, AJ Szabó, Á Vannay
    International Journal of Molecular Sciences, 2022-02-14;23(4):.
    Species: Human
    Sample Types: Serum
  23. Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula
    Authors: S Hakki, EJ Robinson, MG Robson
    Scientific Reports, 2022-01-26;12(1):1427.
    Species: Human
    Sample Types: Plasma
  24. Adiponectin and Stnfr2 peripheral levels are associated with cardiovascular risk in patients with schizophrenia
    Authors: ICS Dias, SM de Campos-, ELM Vieira, APL Mota, PS Azevedo, VTDS Anício, FC Guimarães, LM Mantovani, BF Cruz, AL Teixeira, JV Salgado
    Journal of psychiatric research, 2021-11-09;0(0):.
    Species: Human
    Sample Types: Serum
  25. Gestational Weight Gain Influences the Adipokine-Oxidative Stress Association during Pregnancy
    Authors: JM Solis Pare, O Perichart, A Montoya Es, E Reyes Muño, S Espino Y S, V Ortega Cas, D Medina Bas, M Tolentino, M Sanchez Ma, S Nava Salaz, G Estrada Gu
    Obesity facts, 2021-09-14;0(0):1-9.
    Species: Human
    Sample Types: Serum
  26. Altered cardiometabolic profile in girls with central precocious puberty and adipokines: A propensity score matching analysis
    Authors: JN Zurita-Cru, MA Villasís-K, L Manuel-Apo, L Damasio-Sa, A Gutierrez-, G Wakida-Kus, M Padilla-Ro, C Maldonado-, E Garrido-Ma, AJ Rivera-Her, E Nishimura-
    Cytokine, 2021-07-30;0(0):155660.
    Species: Human
    Sample Types: Serum
  27. The dynamics of human bone marrow adipose tissue in response to feeding and fasting
    Authors: PK Fazeli, MA Bredella, OG Pachon-Peñ, W Zhao, X Zhang, AT Faje, M Resulaj, SP Polineni, TM Holmes, H Lee, EK O'Donnell, OA MacDougald, MC Horowitz, CJ Rosen, A Klibanski
    JCI Insight, 2021-06-22;0(0):.
    Species: Human
    Sample Types: Serum
  28. Predictors of Early-Recurrence Atrial Fibrillation after Catheter Ablation in Women and Men with Abnormal Body Weight
    Authors: J Budzianows, J Hiczkiewic, K ?ojewska, E Kawka, R Rutkowski, K Korybalska
    Journal of Clinical Medicine, 2021-06-18;10(12):.
    Species: Human
    Sample Types: Serum
  29. Impact of the Uncoupling Protein 1 on Cardiovascular Risk in Patients with Rheumatoid Arthritis
    Authors: LI Lyngfelt, MC Erlandsson, M Nadali, S Hedjazifar, R Pullerits, KM Andersson, P Brembeck, ST Silfverswä, U Smith, MI Bokarewa
    Cells, 2021-05-07;10(5):.
    Species: Human
    Sample Types: Serum
  30. Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model
    Authors: H Horder, M Guaza Lash, N Grummel, A Nadernezha, J Herbig, S Ergün, J Te beta mar, J Groll, B Fabry, P Bauer-Krei, T Blunk
    Cells, 2021-04-03;10(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. The effect of Gestational Diabetes Mellitus on the fetal compartment
    Authors: TPB De Luccia, E Ono, R Menon, AU Borbely, R Mattar, L Richardson, ALM da Silva, RM Botelho, MLTLF da Rocha, S Daher
    Journal of reproductive immunology, 2021-03-24;145(0):103314.
    Species: Human
    Sample Types: Serum
  32. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020-11-19;0(0):.
    Species: Human
    Sample Types: Plasma
  33. First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus
    Authors: JHN Schuitemak, RHJ Beernink, A Franx, TIFH Cremers, MPH Koster
    PLoS ONE, 2020-11-18;15(11):e0242423.
    Species: Human
    Sample Types: Serum
  34. The Associations between Dairy Product Consumption and Biomarkers of Inflammation, Adipocytokines, and Oxidative Stress in Children: A Cross-Sectional Study
    Authors: H Aslam, FN Jacka, W Marx, K Karatzi, C Mavrogiann, E Karaglani, M Mohebbi, JA Pasco, A O'Neil, M Berk, T Nomikos, S Kanellakis, O Androutsos, Y Manios, G Moschonis
    Nutrients, 2020-10-06;12(10):.
    Species: Human
    Sample Types: Plasma
  35. BCG vaccination in humans inhibits systemic inflammation in a sex-dependent manner
    Authors: VACM Koeken, LCJ de Bree, VP Mourits, SJ Moorlag, J Walk, B Cirovic, RJ Arts, M Jaeger, H Dijkstra, H Lemmers, LA Joosten, CS Benn, R van Crevel, M Netea
    J. Clin. Invest., 2020-10-01;0(0):.
    Species: Human
    Sample Types: Plasma
  36. Elafin inhibits obesity, hyperglycemia, and liver steatosis in high-fat diet-treated male mice
    Authors: J Wang, C Ortiz, L Fontenot, R Mukhopadhy, Y Xie, IKM Law, DQ Shih, SA Mattai, Z Li, HW Koon
    Sci Rep, 2020-07-30;10(1):12785.
    Species: Human
    Sample Types: Serum
  37. Maternal Anthropometric Factors and Circulating Adipokines as Predictors of Birth Weight and Length
    Authors: D Mazurek, M Bronkowska
    Int J Environ Res Public Health, 2020-07-03;17(13):.
    Species: Human
    Sample Types: Plasma
  38. Deficiency of Urokinase Plasminogen Activator May Impair beta Cells Regeneration and Insulin Secretion in Type 2 Diabetes Mellitus
    Authors: CZ Wu, SH Ou, LC Chang, YF Lin, D Pei, JS Chen
    Molecules, 2019-11-20;24(23):.
    Species: Human
    Sample Types: Serum
  39. Using a cultural dance program to increase sustainable physical activity for breast cancer survivors-A pilot study
    Authors: LWM Loo, K Nishibun, L Welsh, T Makolo, CD Chong, I Pagano, H Yu, EO Bantum
    Complement Ther Med, 2019-09-21;47(0):102197.
    Species: Human
    Sample Types: Plasma
  40. Whey Protein Supplementation Compared to Collagen Increases Blood Nesfatin Concentrations and Decreases Android Fat in Overweight Women: A Randomized Double-Blind Study
    Authors: BM Giglio, RM Schincagli, AS da Silva, ICS Fazani, PA Monteiro, JF Mota, JP Cunha, C Pichard, GD Pimentel
    Nutrients, 2019-09-02;11(9):.
    Species: Human
    Sample Types: Plasma
  41. Growth and differentiation factor 15 is a biomarker for low back pain-associated disability
    Authors: N Tarabeih, A Shalata, S Trofimov, A Kalinkovic, G Livshits
    Cytokine, 2019-02-15;117(0):8-14.
    Species: Human
    Sample Types: Plasma
  42. Effects of the inspiratory muscle training and aerobic training on respiratory and functional parameters, inflammatory biomarkers, redox status and quality of life in hemodialysis patients: A randomized clinical trial
    Authors: PHS Figueiredo, MMO Lima, HS Costa, JB Martins, OD Flecha, PF Gonçalves, FL Alves, VGB Rodrigues, EHB Maciel, VA Mendonça, ACR Lacerda, ÉLM Vieira, AL Teixeira, F de Paula, CH Balthazar
    PLoS ONE, 2018-07-26;13(7):e0200727.
    Species: Human
    Sample Types: Plasma
  43. Impact of rural-urban environment on metabolic profile and response to a 5-day high-fat diet
    Authors: DL Tahapary, K de Ruiter, F Kurniawan, Y Djuardi, Y Wang, SME Nurdin, E Iskandar, D Minggu, E Yunir, B Guigas, T Supali, PCN Rensen, E Sartono, P Soewondo, DS Harbuwono, JWA Smit, M Yazdanbakh
    Sci Rep, 2018-05-25;8(1):8149.
    Species: Human
    Sample Types: Plasma
  44. Nutrients restriction upregulates adiponectin in epicardial or subcutaneous adipose tissue: impact inde novoheart failure patients
    Authors: RM Agra, Á Fernández-, E Díaz-Rodrí, A Cordero, A Varela-Rom, I Gómez-Oter, JNL Canoa, ÁL Fernández, JM Martínez-C, JR González-J, S Eiras
    Int J Med Sci, 2018-02-12;15(5):417-424.
    Species: Human
    Sample Types: Plasma
  45. Using Resistin, glucose, age and BMI to predict the presence of breast cancer
    Authors: M Patrício, J Pereira, J Crisóstomo, P Matafome, M Gomes, R Seiça, F Caramelo
    BMC Cancer, 2018-01-04;18(1):29.
    Species: Human
    Sample Types: Serum
  46. High Expression of STAT3 in Subcutaneous Adipose Tissue Associates with Cardiovascular Risk in Women with Rheumatoid Arthritis
    Authors: M Nadali, R Pullerits, KME Andersson, ST Silfverswä, MC Erlandsson, MI Bokarewa
    Int J Mol Sci, 2017-11-13;18(11):.
    Species: Human
    Sample Types: Serum
  47. Evolution and bad prognostic value of advanced glycation end products after acute heart failure: relation with body composition
    Authors: B Paradela-D, Á Fernández-, D Bou-Teen, S Eiras, R González-F, RM Agra, A Varela-Rom, AI Castro-Pai, MC Carreira, FF Casanueva, E Álvarez, JR González-J
    Cardiovasc Diabetol, 2017-09-15;16(1):115.
    Species: Human
    Sample Types: Plasma
  48. Experimental type 2 diabetes induction reduces serum vaspin, but not serum omentin, in Wistar rats
    Authors: CA Castro, KA da Silva, MM Buffo, KNZ Pinto, FO Duarte, KO Nonaka, FF Aníbal, ACGO Duarte
    Int J Exp Pathol, 2017-04-25;0(0):.
    Species: Human
    Sample Types: Serum
  49. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017-04-11;12(4):e0175109.
    Species: Human
    Sample Types: Serum
  50. Low adiponectin is associated with diastolic dysfunction in women: a cross-sectional study from the Troms� Study
    Authors: JV Norvik, H Schirmer, K Ytrehus, TG Jenssen, SN Zykova, AE Eggen, BO Eriksen, MD Solbu
    BMC Cardiovasc Disord, 2017-03-14;17(1):79.
    Species: Human
    Sample Types: Serum
  51. Improvement of cardiometabolic markers after fish oil intervention in young Mexican adults and the role of PPAR? L162V and PPAR?2 P12A
    Authors: A Binia, C Vargas-Mar, M Ancira-Mor, LM Gosoniu, I Montoliu, E Gámez-Vald, DC Soria-Cont, A Angeles-Qu, R Gonzalez-A, S Fernández, D Martínez-C, B Hernández-, M Ramírez-So, C Pérez-Orte, Y Rodríguez-, I Castan, I Rubio-Alia, F Vadillo-Or, R Márquez-Ve, R Bojalil, JC López-Alva, P Valet, M Kussmann, I Silva-Zole, ME Tejero
    J. Nutr. Biochem., 2017-02-10;43(0):98-106.
    Species: Human
    Sample Types: Serum
  52. Secretory activity of subcutaneous abdominal adipose tissue in male patients with rheumatoid arthritis and osteoarthritis - association with clinical and laboratory data
    Authors: Ewa Kontny
    Reumatologia, 2016-11-28;54(5):227-235.
    Species: Human
    Sample Types: Tissue Explants
  53. Circulating periostin in relation to insulin resistance and nonalcoholic fatty liver disease among overweight and obese subjects
    Sci Rep, 2016-11-25;6(0):37886.
    Species: Human
    Sample Types: Serum
  54. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia
    Authors: E Leinonen
    Eur. Psychiatry, 2016-11-10;40(0):13-19.
    Species: Human
    Sample Types: Serum
  55. The Association Between Adiponectin, Serum Uric Acid and Urinary Markers of Renal Damage in the General Population: Cross-Sectional Data from the Troms� Study
    Kidney Blood Press Res, 2016-09-14;41(5):623-634.
    Species: Human
    Sample Types: Serum
  56. Human adipose tissue conditioned media from lean subjects is protective against H2O2 induced neurotoxicity in human SH-SY5Y neuronal cells.
    Authors: Wan Z, Mah D, Simtchouk S, Kluftinger A, Little J
    Int J Mol Sci, 2015-01-06;16(1):1221-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Expression of obesity markers and Persistent Organic Pollutants levels in adipose tissue of obese patients: reinforcing the obesogen hypothesis?
    Authors: Pereira-Fernandes A, Dirinck E, Dirtu A, Malarvannan G, Covaci A, Van Gaal L, Vanparys C, Jorens P, Blust R
    PLoS ONE, 2014-01-10;9(1):e84816.
    Species: Human
    Sample Types: Serum
  58. Osteocalcin increase after bariatric surgery predicts androgen recovery in hypogonadal obese males.
    Authors: Samavat J, Facchiano E, Cantini G, Di Franco A, Alpigiano G, Poli G, Seghieri G, Lucchese M, Forti G, Luconi M
    Int J Obes (Lond), 2013-12-05;38(3):357-63.
    Species: Human
    Sample Types: Serum
  59. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1.
    Authors: Pellegrinelli V, Rouault C, Veyrie N, Clement K, Lacasa D
    Diabetes, 2013-10-15;63(2):535-49.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Adipokine resistin predicts anti-inflammatory effect of glucocorticoids in asthma.
    Authors: Leivo-Korpela S, Lehtimaki L, Vuolteenaho K, Nieminen R, Kankaanranta H, Saarelainen S, Moilanen E
    J Inflamm (Lond), 2011-05-26;8(0):12.
    Species: Human
    Sample Types: Plasma
  61. LIGHT (TNFSF14) inhibits adipose differentiation without affecting adipocyte metabolism.
    Authors: Tiller G, Laumen H, Fischer-Posovszky P, Finck A, Skurk T, Keuper M, Brinkmann U, Wabitsch M, Link D, Hauner H
    Int J Obes (Lond), 2010-06-15;35(2):208-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Regulation of adipokine production in human adipose tissue by propionic acid.
    Authors: Al-Lahham SH, Roelofsen H, Priebe M, Weening D, Dijkstra M, Hoek A, Rezaee F, Venema K, Vonk RJ
    Eur. J. Clin. Invest., 2010-03-25;40(5):401-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Gene-nutrient interactions in the metabolic syndrome: single nucleotide polymorphisms in ADIPOQ and ADIPOR1 interact with plasma saturated fatty acids to modulate insulin resistance.
    Authors: Ferguson JF, Phillips CM, Tierney AC, Perez-Martinez P, Defoort C, Helal O, Lairon D, Planells R, Shaw DI, Lovegrove JA, Gjelstad IM, Drevon CA, Blaak EE, Saris WH, Leszczynska-Golabek I, Kiec-Wilk B, Riserus U, Karlstrom B, Miranda JL, Roche HM
    Am. J. Clin. Nutr., 2009-12-23;91(3):794-801.
    Species: Human
    Sample Types: Plasma
  64. Adiponectin upregulates monocytic activin A but systemic levels are not altered in obesity or type 2 diabetes.
    Authors: Weigert J, Neumeier M, Wanninger J, Schober F, Sporrer D, Weber M, Schramm A, Wurm S, Stogbauer F, Filarsky M, Schaffler A, Aslanidis C, Scholmerich J, Buechler C
    Cytokine, 2009-01-06;45(2):86-91.
    Species: Human
    Sample Types: Plasma
  65. Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
    Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
    Endocrinology, 2008-12-12;150(4):1688-96.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Reduced response to adiponectin and lower abundance of adiponectin receptor proteins in type 2 diabetic monocytes.
    Authors: Weigert J, Neumeier M, Wanninger J, Wurm S, Kopp A, Schober F, Filarsky M, Schaffler A, Zeitoun M, Aslanidis C, Buechler C
    FEBS Lett., 2008-04-28;582(12):1777-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Isoginkgetin enhances adiponectin secretion from differentiated adiposarcoma cells via a novel pathway involving AMP-activated protein kinase.
    Authors: Liu G, Grifman M, Macdonald J, Moller P, Wong-Staal F, Li QX
    J. Endocrinol., 2007-09-01;194(3):569-78.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Cardiovascular risk factors are reduced with a low dose of acarbose in obese patients with polycystic ovary syndrome.
    Authors: Araujo Penna I, Canella PR, Vieira CS, Silva de Sa MF, dos Reis RM, Ferriani RA
    Fertil. Steril., 2007-04-06;88(2):519-22.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human Adiponectin/Acrp30 DuoSet ELISA
By Anonymous on 12/04/2019
Sample Tested: EDTA Plasma

plasma diluted 1:5000

Human Adiponectin/Acrp30 DuoSet ELISA DY1065
Human Adiponectin/Acrp30 DuoSet ELISA
By Carmen Martin-Ruiz on 08/15/2019
Sample Tested: EDTA Plasma

Used on EDTA Plasma at 1/6000 dilution. Signal at 450nm corrected for signal at 550nm. Very tight replicates, confident results.

Human Adiponectin/Acrp30 DuoSet ELISA DY1065
Human Adiponectin/Acrp30 DuoSet ELISA
By Leslie Priddy on 04/04/2018
Sample Tested: Serum
Human Adiponectin/Acrp30 DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma
Human Adiponectin/Acrp30 DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma
Human Adiponectin/Acrp30 DuoSet ELISA
By Anonymous on 08/03/2017
Sample Tested: Serum and Plasma

Sample dilution used- 1 in 10000 in reagent diluent

Human Adiponectin/Acrp30 DuoSet ELISA DY1065
Human Adiponectin/Acrp30 DuoSet ELISA
By Jonatan Dereke on 05/17/2017
Sample Tested: EDTA Plasma

We ran human plasma at a 1:2000 dilution for this assay.

Human Adiponectin/Acrp30 DuoSet ELISA DY1065