Human Aldo-keto Reductase 1C1/AKR1C1 Antibody

Catalog #: MAB6529 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6529
MAB6529-SP

Detection of Human Aldo‑keto Reductase 1C1/AKR1C1 by Western Blot.

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All Aldo-keto Reductase 1C1/AKR1C1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Aldo-keto Reductase 1C1/AKR1C1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Aldo‑keto Reductase 1C1/AKR1C1 in ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Aldo-keto Reductase 1C3 and 1C4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 859026

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Aldo‑keto Reductase 1C1/AKR1C1
Met1-Tyr323
Accession # Q04828

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Aldo‑keto Reductase 1C1/AKR1C1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Aldo-keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldo-keto Reductase 1C1/AKR1C1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Aldo‑keto Reductase 1C1/AKR1C1 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aldo-keto Reductase 1C1/AKR1C1

AKR1C1 (20-alpha -hydroxysteroid dehydrogenase, 20‑ alpha ‑HSD) is a member of aldo-keto reductase (AKR) superfamily. AKRs perform the NAD(P)H-dependent reduction of carbonyl groups (1). Four AKR1C isoforms (AKR1C1-C4) are known to exist in humans. They are all highly expressed in the liver. Three isoforms, excluding AKR1C4, have a wider expression pattern including prostate, testes, uterus, mammary gland, and haemopoietic progenitors (2). These enzymes are able to accept various natural steroids as substrates, including 3-, 7-, and 20‑ketosteroids (3). They can also activate prodrugs such as synthetic steroid hormone tibolone by converting it into active 3 alpha / beta -hydroxy form (4). They are recognized as phase I drug-metabolizing enzymes involved in the maintenance of steroid homeostasis, prostaglandin metabolism, and metabolic activation of polycyclic aromatic hydrocarbons (5). Their reactions introduce a hydroxyl group into the product making it available for sulfonation and glucuronidation by phase II enzyme. Elevated expression of these enzymes is related to cancer with hormone-dependent malignancies (6, 7). Increased levels of expression of AKR1C1 parallels increased cell proliferation activity in human colon cancer cells. It has been shown to be associated with oncogenic potential and proproliferative effects. It is also involved in cancer cell chemoresistance.

References

Jez, J.M. et al. (1997) Biochem J. 326:499.
Penning, T.M. et al. (2000) Biochem. J. 351:67.
Rizner, T.L. et al. (2003) Endocrinology. 144:2922.
Steckelbroeck, S. et al. (2006) J. Pharmacol. Exp. Ther. 316:1300.
Penning, T.M. et al. (2004) Mol. Cell. Endocrinol. 1784:1342.
Penning, T.M. and M.C. Byrns (2009) Ann. N. Y. Acad. Sci. 1155:33.
Baumann, D.R. et al. (2004) Drug News Perspect. 17:563.

Long Name

Aldo-keto Reductase Family 1, Member C1

Entrez Gene IDs

1645 (Human)

Alternate Names

20 alpha-hydroxysteroid dehydrogenase; 20-ALPHA-HSD; 20-alpha-hydroxysteroid dehydrogenase; 2-ALPHA-HSD; AKR1C1; Aldo-keto Reductase 1C1; aldo-keto reductase family 1 member C1; aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase); AldoketoReductase 1C1; C9; Chlordecone reductase homolog HAKRC; DD1/DD2; DD1MGC8954; DDH1; DDHH-37; dihydrodiol dehydrogenase 1; Dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase isoform DD1; EC 1.1.1; EC 1.1.1.-; EC 1.1.1.112; EC 1.1.1.149,2-ALPHA-HSD; EC 1.3.1.20; HAKRC; HAKRCDDH1aldo-keto reductase C; HBAB; hepatic dihydrodiol dehydrogenase; High-affinity hepatic bile acid-binding protein; Indanol dehydrogenase; MBAB; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase