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Human B7-1/CD80 Antibody

Catalog #: MAB140
25 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB140-SP
MAB140-100
MAB140-500
Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-1/CD80 Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7‑1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 37711
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑1/CD80
Val35-Asn242 (predicted)
Accession # P33681
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
5-25 µg/mL
See below

Human B7-1/CD80 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human B7‑1/CD80 Biotinylated Antibody (Catalog # BAM1402)

Standard: Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF (Catalog # 140-B1)

Neutralization
Measured by its ability to neutralize B7‑1/CD80 and PHA-induced IL‑2 secretion in the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera and 10 µg/mL PHA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7‑1/CD80 antibody in Raji Human Cell Line antibody by Flow Cytometry. View Larger

Detection of B7‑1/CD80 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Neutralization IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. View Larger

IL‑2 secretion Induced by B7‑1/CD80 and Neutralization by Human B7‑1/CD80 Antibody. Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by the Human IL-2 Quantikine ELISA Kit (Catalog # D2050). IL-2 secretion elicited by Recombinant Human B7-1/CD80 Fc Chimera (1 µg/mL) and PHA (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140). The ND50 is typically 0.2-1 µg/mL.

Immunohistochemistry B7-1/CD80 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Dual RNAscope ISH-IHC Compatible B7-1/CD80 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

B7-1/CD80 in Human Tonsil Using Dual RNAscope®ISH and IHC. B7-1/CD80 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 42471) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog# MAB140) at 5ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, brown). Tissues were counterstained with hematoxylin (blue).

Immunohistochemistry Detection of Mouse B7-1/CD80 by Immunohistochemistry View Larger

Detection of Mouse B7-1/CD80 by Immunohistochemistry MNK inhibitors induce polarization of TAMs toward an M2 phenotype in ex vivo human PDAC slice cultures.(A) Serial sections of human PDAC tumors (n = 15) were stained for Arginase-1 and phosphorylated eIF4ES209 (p-eIF4E) by IHC. Scale bar: 200 μm. The staining positivity was analyzed by the TissueGnostics Imaging system using the HistoQuest, and the correlation analysis for Arginase-1 and p-eIF4E expression was performed using GraphPad. (B) Slice cultures (n = 1–4 replicates for each treatment group) from 6 different PDAC tumors were treated with DMSO (vehicle control) or CGP57380 for 5 days and stained for phosphorylated eIF4E (p-eIF4E), CD68, Arginase-1, CD163, MRC1, CD86, CD80, and MHCII. The percentage of positive cells, relative to the total number of nucleated cells, was analyzed by ImageJ. Scale bar: 50 μm. Data points represent individual patients. Paired t test was performed by GraphPad. *P < 0.05; **P < 0.01. (C) The relative expression of MKNK2, CD163, and MRC1 in TCGA studies and their transcript abundance from RNA-Seq data, quantified as RSEM, were downloaded from cBioPortal. Correlation analysis was performed in GraphPad to evaluate the relationship between MKNK2, CD163, and MRC1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35380995), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through Interferon gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% aa sequenceidentity. Human and mouse B7-1 share 44% aa sequenceidentity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Citations for Human B7-1/CD80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Presence of increased inflammatory infiltrates accompanied by activated dendritic cells in the left atrium in rheumatic heart disease
    Authors: Mikio Shiba, Yasuo Sugano, Yoshihiko Ikeda, Hideshi Okada, Toshiyuki Nagai, Hatsue Ishibashi-Ueda et al.
    PLOS ONE
  2. Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia
    Authors: Morten Hjuler Nielsen, Rikke Bæk, Malene Moller Jorgensen, Maiken Mellergaard, Aase Handberg
    Atherosclerosis Plus
  3. The human tissue-biomaterial interface: a role for PPAR gamma -dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype
    Authors: Samuel J. Bullers, Simon C. Baker, Eileen Ingham, Jennifer Southgate
    Tissue Engineering Part A
  4. By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1
    Authors: Patricia Kleinpeter, Christelle Remy-Ziller, Eline Winter, Murielle Gantzer, Virginie Nourtier, Juliette Kempf et al.
    Journal of Virology
  5. The immunophenotype of decidual macrophages in acute atherosis
    Authors: Navleen Gill, Yaozhu Leng, Roberto Romero, Yi Xu, Bogdan Panaitescu, Derek Miller et al.
    American Journal of Reproductive Immunology
  6. B7–1 Is Not Induced in Podocytes of Human and Experimental Diabetic Nephropathy
    Authors: Elena Gagliardini, Rubina Novelli, Daniela Corna, Carlamaria Zoja, Barbara Ruggiero, Ariela Benigni et al.
    Journal of the American Society of Nephrology
  7. Potential Role of Macrophage Polarization in the Progression of Hunner-Type Interstitial Cystitis
    Authors: Ko, KJ;Kim, G;Sung, HH;Park, WY;Lee, KS;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissues
    Applications: IHC
  8. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity
    Authors: TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi
    JCI Insight, 2022-05-09;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. B7-1 and programmed cell death-ligand 1 in primary and lymph node metastasis lesions of non-small cell lung carcinoma
    Authors: T Yamada, Y Miki, M Suzuki, O Kondoh, R Saito-Koya, K Ono, Y Okada, H Sasano
    Cancer Medicine, 2021-12-14;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution
    Authors: Y Liu, S He, XL Wang, W Peng, QY Chen, DM Chi, JR Chen, BW Han, GW Lin, YQ Li, QY Wang, RJ Peng, PP Wei, X Guo, B Li, X Xia, HQ Mai, XD Hu, Z Zhang, YX Zeng, JX Bei
    Nature Communications, 2021-02-02;12(1):741.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. PD-L1 engagement on T cells promotes self-tolerance and suppression of neighboring macrophages and effector T cells in cancer
    Authors: B Diskin, S Adam, MF Cassini, G Sanchez, M Liria, B Aykut, C Buttar, E Li, B Sundberg, RD Salas, R Chen, J Wang, M Kim, MS Farooq, S Nguy, C Fedele, KH Tang, T Chen, W Wang, M Hundeyin, JAK Rossi, E Kurz, MIU Haq, J Karlen, E Kruger, Z Sekendiz, D Wu, SAA Shadaloey, G Baptiste, G Werba, S Selvaraj, C Loomis, KK Wong, J Leinwand, G Miller
    Nat. Immunol., 2020-03-09;21(4):442-454.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: T Cell Activation
  13. The utility of urinary CD80 as a diagnostic marker in patients with renal diseases
    Authors: S Minamikawa, K Nozu, S Maeta, T Yamamura, K Nakanishi, J Fujimura, T Horinouchi, C Nagano, N Sakakibara, H Nagase, H Shima, K Noda, T Ninchoji, H Kaito, K Iijima
    Sci Rep, 2018-11-23;8(1):17322.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Development (Detection)
  14. Comparison of the biological characteristics of human mesenchymal stem cells derived from exfoliated deciduous teeth, bone marrow, gingival tissue, and umbilical cord
    Authors: J Li, SQ Xu, YM Zhao, S Yu, LH Ge, BH Xu
    Mol Med Rep, 2018-09-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis
    Authors: S He, C Wu, J Xiao, D Li, Z Sun, M Li
    Scand. J. Immunol., 2018-04-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Tubular B7-1 expression parallels proteinuria levels, but not clinical outcomes in adult minimal change disease patients
    Authors: SW Lee, SH Baek, JH Paik, S Kim, KY Na, DW Chae, HJ Chin
    Sci Rep, 2017-02-02;7(0):41859.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Autoantibodies against CD80 in patients with COPD
    Authors: Xiongbiao Wang
    Clin Transl Immunology, 2016-10-07;5(10):e103.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  18. Tumor-activated TCRgammadelta(+) T cells from gastric cancer patients induce the antitumor immune response of TCRalphabeta(+) T cells via their antigen-presenting cell-like effects.
    Authors: Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
    J Immunol Res, 2014-03-31;2014(0):593562.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  19. B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production.
    J. Immunol., 2012-06-25;189(3):1202-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012-05-10;119(26):6268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. A synergistic antitumor effect of interleukin-2 addition with CD80 immunogene therapy for peritoneal metastasis of gastric carcinoma.
    Authors: Kosaka K, Yashiro M, Sakate Y, Hirakawa K
    Dig. Dis. Sci., 2007-04-03;52(8):1946-53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Myeloid HO-1 modulates macrophage polarization and protects against ischemia-reperfusion injury
    Authors: Min Zhang, Kojiro Nakamura, Shoichi Kageyama, Akeem O. Lawal, Ke Wei Gong, May Bhetraratana et al.
    JCI Insight
  23. CD80 expression promotes immune surveillance in Barrett’s metaplasia
    Authors: Melania Scarpa, Matteo Fassan, Andromachi Kotsafti, Stefano Realdon, Luigi Dall’Olmo, Tiziana Morbin et al.
    OncoImmunology
  24. Activated macrophages promote invasion by early colorectal cancer via an interleukin 1 beta ‐serum amyloid A1 axis
    Authors: Gota Sudo, Hironori Aoki, Eiichiro Yamamoto, Akira Takasawa, Takeshi Niinuma, Ayano Yoshido et al.
    Cancer Science
  25. PD‐L1 expression, CD8+ and CD4+ lymphocyte rate are predictive of pathological complete response after neoadjuvant chemoradiotherapy for squamous cell cancer of the thoracic esophagus
    Authors: Matteo Fassan, Francesco Cavallin, Vincenza Guzzardo, Andromachi Kotsafti, Melania Scarpa, Matteo Cagol et al.
    Cancer Medicine

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Human B7‑1/CD80 Antibody
By Lu Bai on 10/11/2022
Application: Flow Sample Tested: Dendritic cells Species: Human
Human B7-1/CD80 Antibody
By Anonymous on 04/11/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human B7-1/CD80 Antibody MAB140