Human BLIMP1/PRDM1 Antibody

Catalog # Availability Size / Price Qty
MAB36081
MAB36081-SP
Detection of Human BLIMP1/PRDM1 by Western Blot.
4 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Human BLIMP1/PRDM1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BLIMP1/PRDM1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 646702
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human BLIMP1/PRDM1
Lys667-Cys789
Accession # O75626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
U266 human myeloma cell line or MM.1S human myeloma cell line fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BLIMP1/PRDM1 antibody by Western Blot. View Larger

Detection of Human BLIMP1/PRDM1 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for BLIMP1/PRDM1 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human BLIMP1/PRDM1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human BLIMP1/PRDM1 by Simple WesternTM. Simple Western lane view shows lysates of U266 human myeloma cell line, loaded at 0.2 mg/mL. A specific band was detected for BLIMP1/PRDM1 at approximately 114 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Intracellular Staining by Flow Cytometry View Larger

Detection of BLIMP1/PRDM1 in U266 Human Cell Line by Flow Cytometry. U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Flow (Intracellular) View Larger

Detection of BLIMP1/PRDM1 in MM.1S cells by Flow Cytometry MM.1S cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (Catalog # MAB36081, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BLIMP1/PRDM1

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Long Name
B Lymphocyte Induced Maturation Protein
Entrez Gene IDs
639 (Human); 12142 (Mouse); 309871 (Rat)
Alternate Names
Beta-interferon gene positive regulatory domain I-binding factor; beta-interferon gene positive-regulatory domain I binding factor; BLIMP1; BLIMP-1; BLIMP1MGC118925; blmp-1; B-lymphocyte-induced maturation protein 1; MGC118922; MGC118923; Positive regulatory domain I-binding factor 1; PR domain containing 1, with ZNF domain; PR domain zinc finger protein 1; PR domain-containing protein 1; PRDI-BF1MGC118924; PRDI-binding factor 1; PRDI-binding factor-1; PRDM1; PR-domain zinc finger protein 1; ZNFPR1A1

Product Datasheets

You must select a language.

x

Citations for Human BLIMP1/PRDM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Induced Pluripotent Stem Cells Derived From Two Idiopathic Azoospermia Patients Display Compromised Differentiation Potential for Primordial Germ Cell Fate
    Authors: Fang Fang, Zili Li, Qian Zhao, Zhen Ye, Xiuli Gu, Feng Pan et al.
    Frontiers in Cell and Developmental Biology
  2. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program
    Authors: Yoji Kojima, Chika Yamashiro, Yusuke Murase, Yukihiro Yabuta, Ikuhiro Okamoto, Chizuru Iwatani et al.
    Life Science Alliance
  3. Massively parallel knock-in engineering of human T cells
    Authors: X Dai, JJ Park, Y Du, Z Na, SZ Lam, RD Chow, PA Renauer, J Gu, S Xin, Z Chu, C Liao, P Clark, H Zhao, S Slavoff, S Chen
    Nature Biotechnology, 2023-01-26;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. A Notch/STAT3-driven Blimp-1/c-Maf-dependent molecular switch induces IL-10 expression in human CD4+ T cells and is defective in Crohn�s disease patients
    Authors: J Ahlers, A Mantei, L Lozza, M Stäber, F Heinrich, P Bacher, T Hohnstein, L Menzel, SG Yüz, D Alvarez-Si, AR Bickenbach, C Weidinger, N Mockel-Ten, AA Kühl, B Siegmund, J Maul, C Neumann, A Scheffold
    Mucosal Immunology, 2022-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation
    Authors: D Chen, W Liu, J Zimmerman, WA Pastor, R Kim, L Hosohama, J Ho, M Aslanyan, JJ Gell, SE Jacobsen, AT Clark
    Cell Rep, 2018-12-26;25(13):3591-3602.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Effect of plasma-activated medium on the decrease of tumorigenic population in lymphoma
    Authors: N Wada, JI Ikeda, H Tanaka, H Sakakita, M Hori, Y Ikehara, E Morii
    Pathol. Res. Pract., 2017-04-20;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  7. IMiD immunomodulatory compounds block C/EBP{beta} translation through eIF4E down-regulation resulting in inhibition of MM.
    Authors: Li S, Pal R, Monaghan SA, Schafer P, Ouyang H, Mapara M, Galson DL, Lentzsch S
    Blood, 2011-03-09;117(19):5157-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human BLIMP1/PRDM1 Antibody

There are currently no reviews for this product. Be the first to review Human BLIMP1/PRDM1 Antibody and earn rewards!

Have you used Human BLIMP1/PRDM1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review