Human CD27/TNFRSF7 DuoSet ELISA

Name: Human CD27/TNFRSF7 DuoSet ELISA
Brand: R&D Systems
SKU: DY382-05
Rating: 5 (1 reviews)

Catalog #: DY382-05
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY382-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

Human CD27 / TNFRSF7 ELISA Standard Curve

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All CD27/TNFRSF7 ELISAs

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CD27/TNFRSF7 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

125.0 - 8,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CD27 / TNFRSF7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human CD27 / TNFRSF7 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.

Entrez Gene IDs:

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names:

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

Citations for Human CD27/TNFRSF7 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Soluble CD27 is an intrathecal biomarker of T-cell-mediated lesion activity in multiple sclerosis
Authors: Cencioni, MT;Magliozzi, R;Palmisano, I;Suwan, K;Mensi, A;Fuentes-Font, L;Villar, LM;Fernández-Velasco, JI;Migallón, NV;Costa-Frossard, L;Monreal, E;Ali, R;Romozzi, M;Mazarakis, N;Reynolds, R;Nicholas, R;Muraro, PA;
Journal of neuroinflammation
Species: Human
Sample Types: Cell Culture Supernates, Cerebrospinal Fluid
Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration
Authors: K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López
PLoS ONE, 2020-08-07;15(8):e0236283.
Species: Human
Sample Types: Plasma
Integration of magnetic resonance imaging and protein and metabolite CSF measurements to enable early diagnosis of secondary progressive multiple sclerosis
Authors: S Herman, PE Khoonsari, A Tolf, J Steinmetz, H Zetterberg, T Åkerfeldt, PJ Jakobsson, A Larsson, O Spjuth, J Burman, K Kultima
Theranostics, 2018-08-07;8(16):4477-4490.
Species: Human
Sample Types: CSF
A Predictive 7-Gene Assay and Prognostic Protein Biomarkers for Non-small Cell Lung Cancer
Authors: NL Guo, A Dowlati, RA Raese, C Dong, G Chen, DG Beer, J Shaffer, S Singh, U Bokhary, L Liu, J Howington, T Hensing, Y Qian
EBioMedicine, 2018-06-01;0(0):.
Species: Human
Sample Types: Tissue Homogenates