Human Cerberus 1 Antibody

Catalog #: MAB1075 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1075
MAB1075-SP

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Human Cerberus 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cerberus 1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) DAN, rhGremlin, or recombinant chicken Caronte is observed.

Source

Monoclonal Mouse IgG_2B Clone # 162836

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cerberus 1
Thr18-Ala267 (predicted)
Accession # O95813

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cerberus 1

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Cerberus 1 in Human Liver. Cerberus 1 was detected in immersion fixed paraffin-embedded sections of human liver using 25 µg/mL Mouse Anti-Human Cerberus 1 Monoclonal Antibody (Catalog # MAB1075) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows secondary antibody only control experiment. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1 functions during development by binding to and antagonizing some members of the bone morphogenetic protein (BMP) family.

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Alternate Names

CER1; cerberus 1 (Xenopus laevis) homolog (cysteine knot superfamily); Cerberus 1; cerberus 1, cysteine knot superfamily, homolog (Xenopus laevis); cerberus; cerberus-related 1; Cerberus-related protein; DAN domain family member 4; DAND4; MGC119894; MGC119895; MGC96951