Human COX-2 Antibody Summary
Ala18-Ser112 and Gln386-Leu604
Accession # P35354
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human COX‑2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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COX‑2 in A549 Human Cell Line. COX-2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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COX‑2 in Human Breast Cancer Tissue. COX-2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human COX-2 Monoclonal Antibody (Catalog # MAB4198) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COX-2
Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Over the amino acid range of the immunogen, human COX-2 shows 83% aa identity to mouse COX-2.
Product Datasheets
Citations for Human COX-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Functional Selectivity of Coumarin Derivates Acting via GPR55 in Neuroinflammation
Authors: M Apweiler, J Streyczek, SW Saliba, JA Collado, T Hurrle, S Grä beta le, E Muñoz, C Normann, S Hellwig, S Bräse, BL Fiebich
International Journal of Molecular Sciences, 2022-01-16;23(2):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blots -
Adipose-derived mesenchymal stem cells modulate CD14(++)CD16(+) expression on monocytes from sepsis patients in vitro via prostaglandin E2
Authors: G Qiu, G Zheng, M Ge, L Huang, H Tong, P Chen, D Lai, Y Hu, B Cheng, Q Shu, J Xu
Stem Cell Res Ther, 2017-04-26;8(1):97.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluation of an Aqueous Extract from Horseradish Root (Armoracia rusticana Radix) against Lipopolysaccharide-Induced Cellular Inflammation Reaction
Authors: C Herz, HT Tran, MR Márton, R Maul, S Baldermann, M Schreiner, E Lamy
Evid Based Complement Alternat Med, 2017-01-15;2017(0):1950692.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
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