Human Decorin Antibody Summary
Gly17-Lys172
Accession # NP_598013.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Decorin by Simple WesternTM. Simple Western lane view shows lysates of human uterus tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 145 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Decorin
Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.
- Naito, Z. (2005) J. Nippon Med. Sch. 72:137.
- Matsushima, N. et al. (2005) Cell. Mol. Life Sci. 62:2771.
- Danielson, K. et al. (1993) Genomics 15:146.
Product Datasheets
Citations for Human Decorin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Systemically Administered Homing Peptide Targets Dystrophic Lesions and Delivers Transforming Growth Factor-beta (TGFbeta) Inhibitor to Attenuate Murine Muscular Dystrophy Pathology
Authors: A Iqbal, U May, SN Prince, TAH Järvinen, A Heydemann
Pharmaceutics, 2021-09-18;13(9):.
Species: Human
Sample Types: Recombinant Proteins
Applications: Western Blot -
Regional contribution of proteoglycans to the fracture toughness of the dentin extracellular matrix
Authors: Y Alania, J Creighton, LT Trevelin, CA Zamperini, AK Bedran-Rus
J Biomech, 2020-01-16;0(0):109633.
Species: Human
Sample Types: Complex Sample Type
Applications: IHC -
Conferring extracellular matrix affinity enhances local therapeutic efficacy of anti-TNF-alpha antibody in a murine model of rheumatoid arthritis
Authors: K Katsumata, J Ishihara, K Fukunaga, A Ishihara, E Yuba, E Budina, JA Hubbell
Arthritis Res. Ther., 2019-12-23;21(1):298.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome
Authors: N Chiarelli, G Carini, N Zoppi, M Ritelli, M Colombi
PLoS ONE, 2018-01-18;13(1):e0191220.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dermatan sulfate is a player in the transglutaminase 2 interaction network
Authors: G Wisowski, EM Ko?ma, T Bielecki, A Pude?ko, K Olczyk
PLoS ONE, 2017-02-15;12(2):e0172263.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation -
Proteomics of thyroid tumours provides new insights into their molecular composition and changes associated with malignancy
Authors: J Martínez-A, R Clifton-Bl, MP Molloy
Sci Rep, 2016-03-30;6(0):23660.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
J Bone Miner Res, 2015-05-21;30(8):1445-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Possible dual role of decorin in abdominal aortic aneurysm.
Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu
PLoS ONE, 2015-03-17;10(3):e0120689.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
Authors: Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, Nurnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, Nurnberg P, Sugahara K, Mundlos S, Hoffmann K
Am. J. Hum. Genet., 2011-07-15;89(1):15-27.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Lack of CXC chemokine receptor 3 signaling leads to hypertrophic and hypercellular scarring.
Authors: Yates CC, Krishna P, Whaley D, Bodnar R, Turner T, Wells A
Am. J. Pathol., 2010-03-04;176(4):1743-55.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Role of decorin in the antimyeloma effects of osteoblasts.
Authors: Li X, Pennisi A, Yaccoby S
Blood, 2008-04-24;112(1):159-68.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
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