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Human DLL1 Antibody

Catalog #: MAB1818
3 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1818-SP
MAB1818-500
MAB1818-100
Detection of DLL1 in T98G Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DLL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human DLL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DLL4 is observed.
Source
Monoclonal Mouse IgG2B Clone # 251127
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DLL1
Ser22-Gly540
Accession # AAG09716
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DLL1 (Catalog # 1818-DL) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human DLL1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human DLL1 Biotinylated Antibody (Catalog # BAF1818)

Standard: Recombinant Human DLL1 His-tag Protein, CF (Catalog # 1818-DL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DLL1 antibody in T98G Human Cell Line antibody by Flow Cytometry. View Larger

Detection of DLL1 in T98G Human Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Mouse Anti-Human DLL1 Monoclonal Antibody (Catalog # MAB1818, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

References
  1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
  3. Karanu, F.N. et al. (2001) Blood 97:1960. 
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
  6. Takahashi, Y. et al. (2003) Development 130:4259. 
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
  12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
Long Name
Delta-like 1
Entrez Gene IDs
28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names
delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

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Citations for Human DLL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lutein inhibits proliferation, invasion and migration of hypoxic breast cancer cells via downregulation of HES1
    Authors: Yuan Li, Yuekun Zhang, Xiaosa Liu, Mingchen Wang, Peng Wang, Jianbo Yang et al.
    International Journal of Oncology
  2. The CD46-Jagged1 interaction is critical for human TH1 immunity.
    Authors: Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C
    Nat Immunol, 2012-10-21;13(12):1213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence.
    Authors: Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M
    Int. Immunol., 2006-10-11;18(12):1637-46.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Reviews for Human DLL1 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human DLL1 Antibody
By Anonymous on 12/15/2021
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human
Human DLL1 Antibody
By Jignesh Ganeshbhai Patel on 09/10/2018
Application: WB Sample Tested: Blood mononuclear cells (PBMCs),HeLa cells Species: Human
Human DLL1 Antibody
By Jignesh Patel on 09/10/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human
Human DLL1 Antibody
By Jignesh Patel on 08/21/2018
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human